Rat Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

ELISA: Rat Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP3-18767] - Samples were spiked with high concentrations of Rat Osteoprotegerin/TNFRSF11B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP3-18767] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL

Rat Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat Osteoprotegerin/TNFRSF11B in samples. No significant cross-reactivity or interference between Rat Osteoprotegerin/TNFRSF11B and analogues was observed.
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.15
• Intra-Assay: %CV < 5.57
• Spike Recovery: 90-112%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFRSF11B

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

• OCIF
• OCIFMGC29565
• OPG
• OPGtumor necrosis factor receptor superfamily member 11B
• Osteoclastogenesis inhibitory factor
• Osteoprotegerin
• TNFRSF11B
• TR1
• tumor necrosis factor receptor superfamily, member 11b

Background

Osteoprotegerin is encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Osteoprotegerin/TNFRSF11B ELISA Kit (NBP3-18767). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFRSF11B