Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

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ELISA: Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP2-60100] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma, tissue homogenates
Standard Curve Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml

Order Details

Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of mouse OPG. No significant cross-reactivity or interference between mouse OPG and analogues was observed.
Standard Curve Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV%<10%
Intra-Assay
CV%<8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF11B

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Kit Components

Components
  1. Standard (Freeze dried)
  2. Stop Solution
  3. Sample Diluent
  4. TMB Substrate
  5. Wash Buffer (25 x concentrate)
  6. Assay plate (12 x 8 coated Microwells)
  7. Adhesive Strip (For 96 wells)
  8. Biotin-antibody (100 x concentrate)
  9. Biotin-antibody Diluent
  10. HRP-avidin (100 x concentrate)
  11. HRP-avidin Diluent
  12. Instruction manual

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Mouse Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

  • OCIF
  • OCIFMGC29565
  • OPGtumor necrosis factor receptor superfamily member 11B
  • Osteoclastogenesis inhibitory factor
  • Osteoprotegerin
  • TNFRSF11B
  • TR1
  • tumor necrosis factor receptor superfamily, member 11b

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF11B
Entrez