Osteoprotegerin/TNFRSF11B Antibody (9C2)

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (9C2) [NBP2-76904] - Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (9C2) [NBP2-76904] - ICC staining Osteoproteger in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (9C2) [NBP2-76904] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.

Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (9C2) [NBP2-76904] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.

Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (9C2) [NBP2-76904] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.

Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (9C2) [NBP2-76904] - Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.

Product Discontinued

Osteoprotegerin/TNFRSF11B Antibody (9C2) Summary

• Immunogen: Recombinant protein within Human Osteoprotegerin/TNFRSF11B aa 1-401 / 401. (SwissProt: O00300 Human; SwissProt: O08712 Mouse; SwissProt: O08727 Rat)
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF11B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for Osteoprotegerin/TNFRSF11B Antibody (9C2)

• OCIF
• OCIFMGC29565
• OPG
• OPGtumor necrosis factor receptor superfamily member 11B
• Osteoclastogenesis inhibitory factor
• Osteoprotegerin
• TNFRSF11B
• TR1
• tumor necrosis factor receptor superfamily, member 11b

Background

Osteoprotegerin is encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF11B