Osteoprotegerin/TNFRSF11B Antibody (69127) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 69127
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Osteoprotegerin/TNFRSF11B Antibody (69127) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Osteoprotegerin/TNFRSF11B
  Glu22-Leu401
  Accession # O00300
• Specificity: Detects human Osteoprotegerin/TNFRSF11B in ELISAs. It has been reported (1), and R&D Systems testing has confirmed, that this antibody shows strong cross-reactivity with an unrelated protein, human Carbonic Anhydrase II. In Western blots, this antibody does not cross‑react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD30, rmCD40, rhDR3, rhDR6, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLT beta R, rhNGF R, rmOPG, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF11B
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody (Catalog number BAF805)
  Standard: Recombinant Human Osteoprotegerin/TNFRSF11B (Catalog number 185-OS)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteoprotegerin/TNFRSF11B Antibody (69127) [Unconjugated]

• OCIF
• OCIFMGC29565
• OPG
• OPGtumor necrosis factor receptor superfamily member 11B
• Osteoclastogenesis inhibitory factor
• Osteoprotegerin
• TNFRSF11B
• TR1
• tumor necrosis factor receptor superfamily, member 11b

Background

Osteoprotegerin is a member of the TNF receptor superfamily expressed by many cell types. It exists in soluble monomeric and dimeric forms as a decoy receptor for its ligands, TRANCE and TRAIL. Osteoprotegerin functions as an antagonist to RANKL-induced osteoclastogenesis and bone resorption.

1. Waterman, E.A. et al. (2007) Intl. J. Cancer 121:1958.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Osteoprotegerin/TNFRSF11B Antibody (MAB8051)(5)

Publications using MAB8051

• S Giovannini, G Tinelli, F Biscetti, G Straface, F Angelini, D Pitocco, L Mucci, R Landolfi, A Flex Serum high mobility group box-1 and osteoprotegerin levels are associated with peripheral arterial disease and critical limb ischemia in type 2 diabetic subjects Cardiovasc Diabetol, 2017-08-08;16(1):99. 2017-08-08 [PMID: 28789654] (ELISA Development (Capture), Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)
• Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA. Cytokine, 2006-12-11;36(1):57-68. 2006-12-11 [PMID: 17161616] (ELISA Development, Human)
• Cross SS, Yang Z, Brown NJ, Balasubramanian SP, Evans CA, Woodward JK, Neville-Webbe HL, Lippitt JM, Reed MW, Coleman RE, Holen I Osteoprotegerin (OPG)--a potential new role in the regulation of endothelial cell phenotype and tumour angiogenesis? Int. J. Cancer, 2006-04-15;118(8):1901-8. 2006-04-15 [PMID: 16287088] (IHC-P, Human)
• Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K. J. Rheumatol., 2005-04-01;32(4):713-20. 2005-04-01 [PMID: 15801030] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: TNFRSF11B
• Uniprot:
  • O00300()