Osteoprotegerin/TNFRSF11B Antibody (5G2)

Western Blot: Osteoprotegerin/TNFRSF11B Antibody (5G2) [NBP1-51670] - Western blot analysis using TNFRSF11B mAb against HEK293 (1) and TNFRSF11B-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (5G2) [NBP1-51670] - Immunofluorescence analysis of HL-60 cells using TNFRSF11B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: Osteoprotegerin/TNFRSF11B Antibody (5G2) [NBP1-51670] - Flow cytometric analysis of HL-60 cells using TNFRSF11B mouse mAb (green) and negative control (purple).

Product Discontinued

Osteoprotegerin/TNFRSF11B Antibody (5G2) Summary

• Immunogen: Purified recombinant fragment of human TNFRSF11B expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF11B
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Osteoprotegerin/TNFRSF11B Antibody (5G2)

• OCIF
• OCIFMGC29565
• OPG
• OPGtumor necrosis factor receptor superfamily member 11B
• Osteoclastogenesis inhibitory factor
• Osteoprotegerin
• TNFRSF11B
• TR1
• tumor necrosis factor receptor superfamily, member 11b

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF11B