OSCAR Overexpression Lysate (Native)

Images

 
Western Blot: OSCAR Overexpression Lysate (Adult Normal) [NBL1-13998] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OSCAR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

OSCAR Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC216426 and based on accession number NM_206818. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens osteoclast associated, immunoglobulin-like receptor (OSCAR), transcript variant 1, mRNA.
Gene
OSCAR

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for OSCAR

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for OSCAR Overexpression Lysate (Native)

  • hOSCAR
  • MGC33613
  • OSCAR
  • osteoclast associated receptor OSCAR-S1
  • osteoclast associated receptor OSCAR-S2
  • osteoclast associated, immunoglobulin-like receptor
  • osteoclast-associated immunoglobulin-like receptor
  • Osteoclast-associated receptor
  • PIGR3
  • PIgR-3
  • Poly-Ig receptor 3
  • Polymeric immunoglobulin receptor 3

Background

Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex (LRC) protein family that plays critical roles in the regulation of both innate and adaptive immune responses. Different from the other LRC members, OSCAR expression is detected specifically in preosteoclasts or mature osteoclasts. OSCAR may be an important bone-specific regulator of osteoclast differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol OSCAR
Entrez