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Ornithine Decarboxylase Antibody (2G5)

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Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - Analysis of ODC1 expression in transfected 293T cell line by ODC1 monoclonal antibody (M01), clone 2G5.Lane 1: ODC1 transfected lysate(51.148 KDa).Lane 2: Non-transfected lysate.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - Analysis of ODC1 expression in transfected 293T cell line by ODC1 monoclonal antibody (M01), clone 2G5.Lane 1: ODC1 transfected lysate(51.148 ...read more
Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - Analysis of monoclonal antibody to ODC1 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - ODC1 monoclonal antibody (M01), clone 2G5. Analysis of ODC1 expression in A-431.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - ODC1 monoclonal antibody (M01), clone 2G5. Analysis of ODC1 expression in human spleen.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - ODC1 monoclonal antibody (M01), clone 2G5. Analysis of ODC1 expression in rat brain.
Immunoprecipitation: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - Analysis of ODC1 transfected lysate using anti-ODC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ODC1 MaxPab ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
2G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Ornithine Decarboxylase Antibody (2G5) Summary

Immunogen
ODC1 (AAH25296, 1 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGAGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV
Specificity
ornithine decarboxylase 1
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ODC1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against transfected lysate for WB. It has been used for ELISA.
Publications
Read Publication using H00004953-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ornithine Decarboxylase Antibody (2G5)

  • ODC
  • ODC1
  • ODCEC 4.1.1.17
  • ornithine decarboxylase 1
  • ornithine decarboxylase

Background

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.
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Western Blot: Arginine decarboxylase Antibody [NBP2-15447] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:5000.
Western Blot: Arginine decarboxylase Antibody [NBP2-15447] - A. 30 ug SK-N-SH whole cell lysate/extract 10 % SDS-PAGE ADC antibody dilution: 1:5000
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Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
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Western Blot: iNOS Antibody [NB300-605] - LCA-induced oxidative stress in 4T1 breast cancer cells. The 4T1 cells were treated with LCA for 48 h, then the indicated measurements were performed. The level of iNOS protein was detected by western blotting (n = 3). Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2072-6694/11/9/1255), licensed under a CC-BY licence.
Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Western blot image submitted by a verified customer review.
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PRH2 GST (N-Term) Pro ...
H00005555-P01
SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Ornithine
Decarboxylase Antibod
H00004953-M01
Ornithine Decarboxylase Antibody (2G5)
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IP

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Bioinformatics

Gene Symbol ODC1
Entrez
OMIM
Uniprot