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Ornithine Decarboxylase Antibody (2G5)

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Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - ODC1 monoclonal antibody (M01), clone 2G5. Analysis of ODC1 expression in rat brain.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - ODC1 monoclonal antibody (M01), clone 2G5. Analysis of ODC1 expression in rat brain.
Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - Analysis of monoclonal antibody to ODC1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - ODC1 monoclonal antibody (M01), clone 2G5. Analysis of ODC1 expression in A-431.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - ODC1 monoclonal antibody (M01), clone 2G5. Analysis of ODC1 expression in human spleen.
Western Blot: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - Analysis of ODC1 expression in transfected 293T cell line by ODC1 monoclonal antibody (M01), clone 2G5.Lane 1: ODC1 transfected lysate(51.148 ...read more
Immunoprecipitation: Ornithine Decarboxylase Antibody (2G5) [H00004953-M01] - Analysis of ODC1 transfected lysate using anti-ODC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ODC1 MaxPab ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
2G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Ornithine Decarboxylase Antibody (2G5) Summary

Immunogen
ODC1 (AAH25296, 1 a.a. - 461 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGAGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV
Specificity
ornithine decarboxylase 1
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ODC1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Antibody reactivity against transfected lysate for WB. It has been used for ELISA.
Publications
Read Publication using H00004953-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ornithine Decarboxylase Antibody (2G5)

  • ODCEC 4.1.1.17
  • ornithine decarboxylase 1
  • ornithine decarboxylase

Background

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
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236-EG
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1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
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Western Blot: Arginine decarboxylase Antibody [NBP2-15447] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:5000.
Western Blot: Arginine decarboxylase Antibody [NBP2-15447] - A. 30 ug SK-N-SH whole cell lysate/extract 10 % SDS-PAGE ADC antibody dilution: 1:5000
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Western Blot: c-Fos Antibody [NB110-75039] - Analysis on marmoset brain lysate prepared in Ripa buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000incubated at 4C overnight.
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Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T
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Catalase Antibody
NBP2-24916
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Ornithine
Decarboxylase Antibod
H00004953-M01
Ornithine Decarboxylase Antibody (2G5)
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IP

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Bioinformatics

Gene Symbol ODC1
Entrez
OMIM
Uniprot