Orc2 Antibody (3E11-1G5)

Images

 
Western Blot: Orc2 Antibody (3E11-1G5) [H00004999-M01] - ORC2L monoclonal antibody (M01), clone 3E11-1G5 Analysis of ORC2L expression in Jurkat.
Immunohistochemistry-Paraffin: Orc2 Antibody (3E11-1G5) [H00004999-M01] - Analysis of monoclonal antibody to ORC2L on formalin-fixed paraffin-embedded human spleen tissue.Antibody concentration 5 ug/ml.
Western Blot: Orc2 Antibody (3E11-1G5) [H00004999-M01] - Western Blot analysis of ORC2L expression in Jurkat ( Cat # L017V1 ).
Western Blot: Orc2 Antibody (3E11-1G5) [H00004999-M01] - ORC2L monoclonal antibody (M01), clone 3E11-1G5. Analysis of ORC2L expression in Hela S3 NE.
Sandwich ELISA: Orc2 Antibody (3E11-1G5) [H00004999-M01] - Detection limit for recombinant GST tagged ORC2L is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, S-ELISA
Clone
3E11-1G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Orc2 Antibody (3E11-1G5) Summary

Immunogen
ORC2L (AAH14834, 1 a.a. ~ 577 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEEDDQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKSVSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSESEYSASNSEDDEGVAQEHEEDTNAVIFSQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYLIASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA
Localization
Nuclear
Marker
Chromatin Marker
Specificity
ORC2L - origin recognition complex, subunit 2-like (yeast)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ORC2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Control
Orc2 Knockout HeLa Cell Lysate
Publications
Read Publication using H00004999-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Orc2 Antibody (3E11-1G5)

  • ORC2Lorigin recognition complex subunit 2
  • origin recognition complex protein 2 homolog
  • origin recognition complex, subunit 2 (yeast homolog)-like
  • origin recognition complex, subunit 2 homolog (yeast)
  • origin recognition complex, subunit 2 homolog
  • origin recognition complex, subunit 2
  • origin recognition complex, subunit 2-like (yeast)
  • origin recognition complex, subunit 2-like

Background

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3L, -4L, and -5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Orc2 Antibody (H00004999-M01)(1)

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ORC2
Entrez
OMIM
Uniprot