OCT1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-56190] - Staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.

ChIP-Exo-Seq composite graph for Anti-POU2F1 (NBP2-56190) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

OCT1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit OCT1 Antibody - BSA Free (NBP2-56190) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: AQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSL
• Predicted Species: Mouse (93%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POU2F1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  OCT1 Recombinant Protein Antigen (NBP2-56190PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for OCT1 Antibody - BSA Free

• FLJ42836
• NF-A1
• Oct-1
• OCT1class 2, transcription factor 1
• OTF1
• POU class 2 homeobox 1
• POU domain class 2, transcription factor 1
• POU2F1

Background

Oct1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR and interacts with NR3C1, AR and PGR. Oct1 is Ubiquitous and belongs to the POU transcription factor family class 2 subfamily. Oct1 contains 1 homeobox domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU2F1