Oct-3/4 Antibody (EM92) - BSA Free

Western Blot: OCT3/4 Antibody (EM92) [NBP2-00352] - F9 mouse embryonal carcinoma cell line (left) and mouse splenocyte (right) lysates were loaded at 1x10e5 cells/lane, probed with 2 ug/mL of Anti-Mouse OCT3/4 Purified and revealed with Anti-Rat IgG HRP.

• Reactivity: Mu
• Applications: WB
• Clone: EM92
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free

Oct-3/4 Antibody (EM92) - BSA Free Summary

• Description: Novus Biologicals Rat Oct-3/4 Antibody (EM92) - BSA Free (NBP2-00352) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody is OCT3/4, Pou5f1, transcription factor 1.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: POU5F1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Oct-3/4 Antibody (EM92) - BSA Free

• 3-Oct
• Oct-3/4
• OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
• Oct4
• Otf3g
• Otf4
• Pou5f1

Background

The EM92 monoclonal antibody reacts with mouse and human Oct-3/4, encoded by the Pou5F1 gene. Oct-3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct-3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct-3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct-3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct-3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Oct-3/4 Antibody (NBP2-00352). (Showing 1 - 1 of 1 FAQ).

1. For the following product, could you let us know the buffer composition? #NBP2-00352, OCT3/4 Antibody (EM92), Preservative: 0.09% Sodium Azide
   • Our OCT3/4 antibody with catalogue number NBP2-00352 is supplied in PBS with 0.09% sodium azide.

Bioinformatics

• Gene Symbol: POU5F1