Oct-3/4 Antibody (240408) [Unconjugated]

NTERA-2 cells were stained with Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Western blot shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct-3/4 Monoclonal Antibody (Catalog # MAB1759) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Oct-3/4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Oct‑3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using Rhodamine Red-coupled anti-rat IgG (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Pertussis toxin does not affect the maintenance of pluripotency pluripotent stem cell colonies or the ability to form all three embryonic germ layers.(A) Pertussis toxin does not affect the maintenance of pluripotency of hES or hiPS cells as assessed by the expression of the pluripotency markers Nanog, Oct4, Sox2, TDGF1, and ZFP42 by quantitative real-time PCR. (B) Immunocytochemistry of TRA-1-81 (green) and Oct-3/4 (red) also did not reveal differences between hES or hiPS cells treated with pertussis toxin and control treatments in pluripotent stem cell colonies. Nuclei were stained with Hoechst (blue). Scale bar, 200 µm. (C) hES or iPS cells treated with pertussis toxin formed embryoid bodies and differentiated into cell types of the all three embryonic germ layers as assessed by immunocytochemistry for alpha -fetoprotein (a marker of endoderm), alpha -smooth muscle actin (mesoderm) and beta III-tubulin (ectoderm). Scale bar, 100 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19936228), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of DFX on spherogenicity of human cancer cell lines and treatment with Nanog siRNA in vitro. (A) After treatment with 0.2% DMSO or 50 μM DFX, a single suspension of HSC-2 cells or OE33 cells was used for the sphere formation assay in a 96-well ultra-low attachment plate. DFX suppressed the spherogenicity of HSC-2 cells and OE33 cells. (B) A single suspension of HSC-2 cells or OE33 cells as described above was used for the spheroid colony assay in a 24-well ultra-low attachment plate. The number of spheres over 50 μm in diameter was counted. The experiments were performed in triplicate, and means ± S.E.M. of each group are shown. DFX significantly suppressed the number of spheres. * p < 0.05. (C) HSC-2 cells were transfected with control or si-Nanog for 48 h, and the expression of stemness markers (Nanog, Sox2, Oct3/4, Klf4, c-Myc) was determined with western blot analysis. beta -actin was used as a loading control. siRNA suppressed the expression of Nanog, Oct3/4, and Klf4. (D) HSC-2 cells were transfected with control or si-Nanog for 48 h, and the sphere formation assay was performed. No differences were found in spherogenicity between the control and si-Nanog cultures. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30717462), licensed under a CC-BY license. Not internally tested by R&D Systems.

Undifferentiated H7 hESC colonies (on passage 15) 3 days after passaging on hFF feeder cells (a) and after 55 passages on MEF feeder cells (b). H7 cells passaged 11 times on hFF feeder cells expressed Nanog (c and d), Oct4 (e and f), SSEA4 (g and h), and TRA-1-60 (i and j). H7 cells on passage 45 on MEF feeder cells expressed Nanog (k and l), Oct4 (m and n), and SSEA4 (o and p). Scale bars 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22315618), licensed under a CC-BY license. Not internally tested by R&D Systems.

Undifferentiated H7 hESC colonies (on passage 15) 3 days after passaging on hFF feeder cells (a) and after 55 passages on MEF feeder cells (b). H7 cells passaged 11 times on hFF feeder cells expressed Nanog (c and d), Oct4 (e and f), SSEA4 (g and h), and TRA-1-60 (i and j). H7 cells on passage 45 on MEF feeder cells expressed Nanog (k and l), Oct4 (m and n), and SSEA4 (o and p). Scale bars 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22315618), licensed under a CC-BY license. Not internally tested by R&D Systems.

The effect of DFX against stemness of miPS cells in vitro and cytotoxicity analysis. (A) miPS cells were treated with the indicated dose of DFX (0, 1, 10, 50, 100 μM) and subjected to western blot analysis with antibodies to stemness markers (Nanog, Sox2, Oct3/4, Klf4, c-Myc) or beta -actin (loading control). Stemness markers were suppressed by DFX at concentrations over 50 μM. (B) miPS cells treated with 50 μM DFX were cultured in suspension for 72 h. DFX treatment of miPS cells suppressed spherogenesis and GFP expression, which indicates suppression of Nanog. (C) Micrographs of the fluorescence-based Live/Dead assay showing live and dead miPS cells following treatment with 0.2% DMSO (control) or 50 μM DFX (magnification ×40). The morphology of miPS cells after treatment with DFX changed from round to spindle shaped. Almost all cells were stained green, which indicates live cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30717462), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clone: 240408
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Oct-3/4 Antibody (240408) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Oct‑3/4
  Met1-Asn265 (Met262Leu)
  Accession # Q01860
• Specificity: Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: POU5F1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody (240408) [Unconjugated]

• 3-Oct
• Oct-3/4
• OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
• Oct4
• Otf3g
• Otf4
• Pou5f1

Background

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
3. Rosner, M.H. et al. (1990) Nature 345:686.
4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oct-3/4 Antibody (MAB1759)(50)

Publications using MAB1759

• Bai, R;Zhang, S;Gu, X;You, Y;Liu, X; Establishment of a TRPV2 knockout human embryonic stem cell line (WAe009-A-1Y) using episomal vector-based CRISPR/Cas9 Stem cell research 2025-05-30 [PMID: 40483902] (Immunocytochemistry, Human)
• Pan, T;Lin, G;Li, X;VanHeyningen, D;Walker, JC;Kohli, S;Saravanan, A;Kondur, A;Jaklic, DC;Pantoja-Gutierrez, S;Vaid, S;Sturza, J;Inoki, K;Imamichi, T;Chang, W;Dang, LT; Delayed forebrain excitatory and inhibitory neurogenesis in STRADA-related megalencephaly via mTOR hyperactivity bioRxiv : the preprint server for biology 2025-05-14 [PMID: 40462897] (Immunocytochemistry, Human)
• Talanaite, D;Wang, H;Qi, M;Lan, F; Generation of a human embryonic stem cell line (WAe009-A-3B) carrying homozygous TNNT2 gene knockout by CRISPR/Cas9 editing Stem cell research 2025-05-03 [PMID: 40344932] (Immunocytochemistry, Human)
• Rakotomamonjy, J;Rylaarsdam, L;Fares-Taie, L;McDermott, S;Davies, D;Yang, G;Fagbemi, F;Epstein, M;Fairbanks-Santana, M;Rozet, JM;Guemez-Gamboa, A; PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model Cell reports 2023-07-20 [PMID: 37480564] (ICC, Human)
• X Wang, J Sun, C Fu, C Liu Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9 Stem Cell Research, 2023-03-21;69(0):103077. 2023-03-21 [PMID: 36965407] (Flow Cytometry, ICC, Human)
• T Ding, Z Gao, H Liu, Y Li, S Liu, L Jian Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system Stem Cell Research, 2023-01-16;67(0):103029. 2023-01-16 [PMID: 36724553] (Flow Cytometry, Human)
• M Zhou, P Huang, R Bai, X Liu Generation of a homozygous TAZ knockout hESCs line by CRISPR/Cas9 system Stem Cell Research, 2022-09-20;64(0):102923. 2022-09-20 [PMID: 36219982] (ICC, Human)
• Julie Bejoy, Justin M. Farry, Jennifer L. Peek, Mariana C. Cabatu, Felisha M. Williams, Richard C. Welch et al. Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease Stem Cell Research & Therapy 12/1/2022 [PMID: 35883199]
• S Farahi, S Hosseini, H Ghanbarian, SM Hashemi, M Salehi, S Hosseini The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level Stem Cells International, 2022-02-15;2022(0):9346767. 2022-02-15 [PMID: 35371264] (ICC/IF, Mouse)
• Miki Nishio, Takuya Matsuura, Shunya Hibi, Shiomi Ohta, Chio Oka, Noriaki Sasai et al. Heterozygous loss of Zbtb38 leads to early embryonic lethality via the suppression of Nanog and Sox2 expression Cell Proliferation 4/1/2022 [PMID: 35297517]
• K Kwa?niak, J Czarnik-Kw, K Malysheva, K Pogoda, O Korchynsky, P Rybojad, B Karczmarek, J Tabarkiewi LncCDH5-3:3 Regulates Apoptosis, Proliferation, and Aggressiveness in Human Lung Cancer Cells Cells, 2022-01-23;11(3):. 2022-01-23 [PMID: 35159188] (Western Blot, Human)
• SK Hota, KS Rao, AP Blair, A Khalilimey, KM Hu, R Thomas, K So, V Kameswaran, J Xu, BJ Polacco, RV Desai, N Chatterjee, A Hsu, JM Muncie, AM Blotnick, SAB Winchester, LS Weinberger, R Hüttenhain, IS Kathiriya, NJ Krogan, JJ Saucerman, BG Bruneau Brahma safeguards canalization of cardiac mesoderm differentiation Nature, 2022-01-26;602(7895):129-134. 2022-01-26 [PMID: 35082446] (ICC, Mouse)
• S Zhang, T Guo, Y Song, M Jiang, Y Jiang, T Dong, S Zhang, H Wang, X Hou Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system Stem Cell Research, 2021-12-20;59(0):102631. 2021-12-20 [PMID: 34953326] (Flow Cytometry, ICC, Human)
• Julie Bejoy, Eddie Spencer Qian, Lauren Elizabeth Woodard Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells STAR Protocols 12/1/2021 [PMID: 34746862]
• S Ma, Q Xu, R Bai, T Dong, Z Peng, X Liu Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing Stem Cell Research, 2021-07-19;55(0):102470. 2021-07-19 [PMID: 34352617] (ICC, Human)
• X Liu, S Zhang, Y Chang, F Wu, R Bai Establishment of a KCNQ1 homozygous knockout human embryonic stem cell line by episomal vector-based CRISPR/Cas9 system Stem Cell Research, 2021-07-22;55(0):102467. 2021-07-22 [PMID: 34343827] (Flow Cytometry, ICC, Human)
• W Wang, R Bai, X Song Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system Stem Cell Research, 2021-07-20;55(0):102471. 2021-07-20 [PMID: 34332328] (Flow Cytometry, ICC, Human)
• A Getachew, Z Yang, X Huang, F Wu, YY Liu, C Yan, F Yang, Y Li Generation of a TLR2 homozygous knockout human embryonic stem cell line WAe001-A-64 using CRISPR/Cas9 editing Stem Cell Research, 2021-05-21;54(0):102401. 2021-05-21 [PMID: 34058685] (Flow Cytometry, Human)
• T Zhao, R Bai, F Wu, WJ Lu, J Zhang Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing Stem Cell Research, 2021-01-06;51(0):102156. 2021-01-06 [PMID: 33497883] (Flow Cytometry, ICC, Human)
• L Andreasson, H Evenbratt, R Mobini, S Simonsson Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces J Biotechnol, 2020-11-02;0(0):. 2020-11-02 [PMID: 33147515] (Bioassay, ICC, Human)
• F Wu, X Li, R Bai, Y Li, J Gao, F Lan Generation of a Junctophilin-2 homozygous knockout human embryonic stem cell line (WAe009-A-36) by an episomal vector-based CRISPR/Cas9 system Stem Cell Res, 2020-08-03;48(0):101930. 2020-08-03 [PMID: 32777767] (ICC, Human)
• L Wei, J Zhang, D Chen, L Feng, C Wu, R Wang, X Li Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi005-A) from a Wilson&#039;s disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene Stem Cell Res, 2020-07-15;47(0):101909. 2020-07-15 [PMID: 32745714] (ICC, Human)
• L Wei, J Zhang, D Chen, L Feng, C Wu, R Wang, X Li Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi002-A) from a patient with spinocerebellar ataxia type 3 Stem Cell Res, 2020-06-29;47(0):101898. 2020-06-29 [PMID: 32650241] (ICC, Human)
• Collins D, Hapke J, Aravalli R, Steer C In vitro Differentiation of TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells HMER 6/1/2020 [PMID: 32607015]
• G Kaufman, NM Kiburi, D Skrtic The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer Biosci. Rep., 2020-06-26;40(6):. 2020-06-26 [PMID: 32495822] (ICC, Human)
• J Zhang, L Wei, R Wang, X Li, Z Liu Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi001-A) from a healthy male individual Stem Cell Res, 2020-04-24;45(0):101809. 2020-04-24 [PMID: 32361313] (ICC, Human)
• J Zhang, L Wei, D Chen, L Feng, C Wu, R Wang, X Li, H Liu Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi004-A) from a Wilson&#039;s disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene Stem Cell Res, 2020-02-20;44(0):101741. 2020-02-20 [PMID: 32113134] (ICC, Human)
• X Guo, Y Tang, P Zhang, S Li, Y Chen, B Qian, H Shen, N Zhao Effect of ectopic high expression of transcription factor OCT4 on the &quot;stemness&quot; characteristics of human bone marrow-derived mesenchymal stromal cells Stem Cell Res Ther, 2019-06-03;10(1):160. 2019-06-03 [PMID: 31159871] (ICC, Human)
• Katsura Y, Ohara T, Noma K et al. A Novel Combination Cancer Therapy with Iron Chelator Targeting Cancer Stem Cells via Suppressing Stemness Cancers (Basel) 2/3/2019 [PMID: 30717462]
• N Ito, K Katoh, H Kushige, Y Saito, T Umemoto, Y Matsuzaki, H Kiyonari, D Kobayashi, M Soga, T Era, N Araki, Y Furuta, T Suda, Y Kida, K Ohta Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency Sci Rep, 2018-01-26;8(1):1634. 1/26/2018 [PMID: 29374279]
• G Xu, D Guo, F Wu, N Abbas, K Lai, F Yuan, K You, Y Liu, Y Zhuang, Y Wu, Y Xu, Y Chen, F Yang, T Pan, YX Li Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing Stem Cell Res, 2017-12-13;27(0):38-41. 2017-12-13 [PMID: 29310060] (ICC, Human)
• F Wu, G Gao, T Pan, Z Yang, G Xu, N Abbas, Y Liu, Y Chen, S Tan, K You, X Ke, Y Zhuang, X Lin, F Yang, YX Li Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing Stem Cell Res, 2017-12-05;27(0):5-9. 2017-12-05 [PMID: 29278761] (ICC, Human)
• Takayuki Ninomiya, Toshiaki Ohara, Kazuhiro Noma, Yuki Katsura, Ryoichi Katsube, Hajime Kashima et al. Iron depletion is a novel therapeutic strategy to target cancer stem cells Oncotarget 11/17/2017 [PMID: 29228699]
• Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9 Stem Cell Res, 2017-05-19;22(0):29-32. 2017-05-19 [PMID: 28952928] (ICC, Human)
• G Kaufman, D Skrtic Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix Tissue Cell, 2017-04-10;0(0):. 2017-04-10 [PMID: 28457531] (ICC, Mouse)
• P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017-04-25;8(17):28074-28092. 2017-04-25 [PMID: 28427209] (ICC, Western Blot, Human)
• Tomomi Kotoku, Koji Kosaka, Miki Nishio, Yasumasa Ishida, Masashi Kawaichi, Eishou Matsuda CIBZ Regulates Mesodermal and Cardiac Differentiation of by Suppressing T and Mesp1 Expression in Mouse Embryonic Stem Cells Scientific Reports 9/23/2016 [PMID: 27659197]
• Zangiacomi Vincent, Kenichi Urakami, Koji Maruyama, Ken Yamaguchi, Masatoshi Kusuhara CD133-positive cancer stem cells from colo205 human colon adenocarcinoma cell line show resistance to chemotherapy and display a specific metabolomic profile Genes & Cancer 7/27/2014 [PMID: 25221643]
• Hagiwara , Kunie, Obayashi , Takeshi, Sakayori , Nobuyuki, Yamanishi , Emiko, Hayashi , Ryuhei, Osumi , Noriko, Nakazawa , Toru, Nishida , Kohji Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells. PLoS ONE, 2014-01-20;9(1):e84072. 2014-01-20 [PMID: 24465393] (ICC, Mouse)
• Cortes-Dericks , Lourdes, Yazd , Ehsan Fa, Mowla , Seyed J, Schmid , Ralph A, Karoubi , Golnaz Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis. Anticancer Res, 2013-12-01;33(12):5365-73. 2013-12-01 [PMID: 24324071] (Western Blot, Human)
• Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells. Stem Cells Int, 2012-01-19;2012(0):875059. 2012-01-19 [PMID: 22315618] (ICC, Human)
• Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells. Mol. Ther., 2010-11-30;19(3):584-93. 2010-11-30 [PMID: 21119622] (ICC, Human)
• Klim JR, Li L, Wrighton PJ A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods, 2010-11-14;7(12):989-94. 2010-11-14 [PMID: 21076418] (ICC, Human)
• Angel M, Yanik MF Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS ONE, 2010-07-23;5(7):e11756. 2010-07-23 [PMID: 20668695] (ICC, Human)
• Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol, 2009-12-24;202(2):193.e1-193.e1. 2009-12-24 [PMID: 20035913] (IHC, Human)
• Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Cell Stem Cell, 2009-04-03;4(4):359-69. 2009-04-03 [PMID: 19341625] (ICC, Mouse)
• Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis. Cancer Res., 2008-08-15;68(16):6533-40. 2008-08-15 [PMID: 18701476] (IHC-Fr, Mouse)
• Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays. Stem Cells, 2008-07-31;26(10):2586-94. 2008-07-31 [PMID: 18669905] (ICC, Mouse)
• Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Stem Cells, 2008-03-06;26(6):1436-43. 2008-03-06 [PMID: 18323409] (ICC, Human)
• Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 2007-02-01;25(2):490-9. 2007-02-01 [PMID: 17284653] (Flow Cytometry, Human, Primate - Macaca mulatta (Rhesus Macaque))

Bioinformatics

• Gene Symbol: POU5F1
• Uniprot:
  • Q01860()