OATP1B1/OATP2 Antibody (ESL)

• Reactivity: Hu, Ca(-), Mu(-), Rt(-)
• Applications: WB, Flow, IHC, IHC-Fr, IHC-P, IP, ICC/IF
• Clone: ESL
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OATP1B1/OATP2 Antibody (ESL) Summary

• Immunogen: Synthetic peptide corresponding to residues E(671) S L N K N K H F V P S A G A D S E T H C(691) of human OATP2.
• Localization: Cell Membrane; multi pass membrane protein
• Specificity: OATP2 (ESL)
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLCO1B1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Immunofluorescence 1:100
• Application Notes: Flow usage was reported in scientific literature (PMID: 25916617). IF: Staining of OATP2 in human liver samples results in a pattern consistent with plasma membrane staining.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for OATP1B1/OATP2 Antibody (ESL)

• HBLRR
• Liver-specific organic anion transporter 1
• LST1
• LST1SLC21A6
• LST-1Solute carrier family 21 member 6
• OATP1B1
• OATP1B1Sodium-independent organic anion-transporting polypeptide 2
• OATP2
• OATP2MGC133282
• OATPC
• OATP-COATP-2
• OATPCsolute carrier organic anion transporter family member 1B1
• SLC01B1
• SLC21A6
• SLCO1B1
• SO1B1
• solute carrier family 21 (organic anion transporter), member 6
• solute carrier organic anion transporter family, member 1B1

Background

The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OATP1B1/OATP2 Antibody (NB100-74481)(3)

Publications using NB100-74481

• Hogg K, Thomas J, Ashford D et al. Quantification of proteins by flow cytometry: Quantification of human hepatic transporter P-gp and OATP1B1 using flow cytometry and mass spectrometry Methods 2015 Apr 24 [PMID: 25916617] (FLOW, Human)
• Yamaguchi H, Okada M, Akitaya S, Ohara H, Mikkaichi T, Ishikawa H, Sato M, Matsuura M, Saga T, Unno M, Abe T, Mano N, Hishinuma T, Goto J. Transport of fluorescent chenodeoxycholic acid via the human organic anion transporters OATP1B1 and OATP1B3. J Lipid Res47(6):1196-202. 2006 Jun. [PMID: 16534140] (WB)
• Cui Y, Konig J, Nies AT et al. Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma. Lab Invest. 2003 Apr. [PMID: 12695556]

Bioinformatics

• Gene Symbol: SLCO1B1
• Entrez:
  • 10599(Human)