OAT Antibody (HL2088) - Azide and BSA Free

Western Blot: OAT Antibody (HL2088) - Azide and BSA Free [NBP3-25585] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [HL2088] (NBP3-25585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: OAT Antibody (HL2088) - Azide and BSA Free [NBP3-25585] - OAT antibody [HL2088] detects OAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. OAT stained by OAT antibody [HL2088] (NBP3-25585) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: OAT Antibody (HL2088) - Azide and BSA Free [NBP3-25585] - OAT antibody [HL2088] detects OAT protein at mitochondria and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: OAT stained by OAT antibody [HL2088] (NBP3-25585) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.

Immunohistochemistry-Paraffin: OAT Antibody (HL2088) - Azide and BSA Free [NBP3-25585] - OAT antibody [HL2088] detects OAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. OAT stained by OAT antibody [HL2088] (NBP3-25585) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL2088
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

OAT Antibody (HL2088) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit OAT Antibody (HL2088) - Azide and BSA Free (NBP3-25585) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human OAT
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: OAT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for OAT Antibody (HL2088) - Azide and BSA Free

• GACR
• HOGA
• OAT ornithine aminotransferase
• OATASE
• OKT

Background

OAT, also known as mitochondrial enzyme ornithine aminotransferase, is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. ( L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.) Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Recombinant OAT protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OAT