NUP188 Antibody - BSA Free

Western Blot: NUP188 Antibody [NBP1-28748] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28748 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28717, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Western Blot: NUP188 Antibody [NBP1-28748] - Full induction of p21 is Nup155-dependent. a Protein fold-changes (blue & grey dots) including p53 targets (red dots) upon Nup155 (orange dot) knockdown compared to the control siRNA (AS) condition are shown as log2 ratios. p21 is highlighted in green as a key effector of the p53 response. Overall 3523 proteins could be quantified by quantitative mass spectrometry in HepG2 cells treated with Nutlin-3a for 24 h. b HepG2 cells were treated either with control siRNA (AS) or three different Nup155 siRNAs (Nup155#1, Nup155#2, & Nup155#3) for 72 h & p53 was induced by adding Nutlin-3a for 24 h. Cell extracts were analysed by immunoblotting with indicated antibodies (upper panel). Densitometric quantification of immunoblots derived from three independent experiments is shown in the lower panel & normalised to the control siRNA (AS) condition. c HepG2 cells were treated either with control siRNA (AS) or two different Nup35, Nup93, & Nup188 siRNAs (Nup35#1, Nup35#2, Nup93#1, Nup93#2, Nup188#1, & Nup188#2) for 72 h. Cells were harvested upon 24 h of Nutlin-3a treatment & extracts were analysed by immunoblotting with the indicated antibodies. For corresponding densitometric analyses see Supplementary Figure 1B-D. d Protein fold-changes of NPC components (purple dots) upon Nup155 knockdown (orange dot) corresponding to the conditions described in a. e Representative confocal microscopy pictures of MAb414 immunofluorescence staining of HepG2 cells treated as indicated. DAPI was used for DNA labeling. Scale bar = 10 µm. *p < 0.05, **p < 0.01, ***p < 0.001 (Student′s t-test). Data are presented as mean ± stdv. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31089132), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NUP188 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1600 and 1650 of human nucleoporin 188kDa using the numbering given in entry NP_056169.1 (GeneID 23511).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUP188
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NUP188 Antibody - BSA Free

• FLJ21639
• KIAA0169hNup188
• nucleoporin 188kDa
• nucleoporin NUP188 homolog

Background

Nucleoporin 188 kDa (NUP188) is one of many nucleoporin proteins that make up the nucleoporin complex which function reside in the nuclear membrane to form nuclear pore structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NUP188 Antibody (NBP1-28748)(2)

Publications using NBP1-28748

• Woo T, Takeo Y, Harwood M et al. The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection Science Advances 2025-10-24 [PMID: 41124246]
• Holzer K, Ori A, Cooke A Nucleoporin Nup155 is part of the p53 network in liver cancer Nat Commun 2019-05-14 [PMID: 31089132] (WB, Human)

Bioinformatics

• Gene Symbol: NUP188
• Entrez:
  • 23511(Human)
• Uniprot:
  • NP_056169.1(Human)