NUP133 Antibody (3E8)

Images

 
Western Blot: NUP133 Antibody (3E8) [H00055746-M01] - NUP133 monoclonal antibody (M01), clone 3E8 Analysis of NUP133 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: NUP133 Antibody (3E8) [H00055746-M01] - Immunofluorescence of monoclonal antibody to NUP133 on HeLa cell. [antibody concentration 10 ug/ml]
Immunohistochemistry-Paraffin: NUP133 Antibody (3E8) [H00055746-M01] - Analysis of monoclonal antibody to NUP133 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
ELISA: NUP133 Antibody (3E8) [H00055746-M01] - Detection limit for recombinant GST tagged NUP133 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
3E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NUP133 Antibody (3E8) Summary

Immunogen
NUP133 (NP_060700, 1069 a.a. ~ 1156 a.a) partial recombinant protein with GST tag.LKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQ
Specificity
NUP133 (3E8)
Isotype
IgG Mix Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NUP133
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using H00055746-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NUP133 Antibody (3E8)

  • FLJ10814
  • hNUP133,133 kDa nucleoporin
  • MGC21133
  • nuclear pore complex protein Nup133
  • nucleoporin 133kD
  • nucleoporin 133kDa
  • Nucleoporin Nup133

Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NUP133 Antibody (H00055746-M01)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NUP133
Entrez
OMIM
Uniprot