Nup53 Antibody

Images

 
Biological Strategies: Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunocytochemistry/ Immunofluorescence: Nup53 Antibody [NB100-93322] - Showing nuclear co-localization with p300 in PANC-1 cells using anti-Nup53 antibody (green). Image from verified customer review.
Immunohistochemistry: Nup53 Antibody [NB100-93322] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NUP35 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: Nup53 Antibody [NB100-93322] - Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Nup53 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 276 and 376 of human nucleoporin 35kDa using the numbering given in entry NP_612142.2 (GeneID 129401).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUP35
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:250- 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-93322 in the following applications:

Publications
Read Publication using NB100-93322.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nup53 Antibody

  • 35 kDa nucleoporin
  • Mitotic phosphoprotein 44
  • MP-44
  • MP44NUP53
  • NP44
  • Nuclear pore complex protein Nup53
  • nucleoporin 35kDa
  • Nucleoporin Nup35
  • nucleoporin NUP53

Background

This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for Nup53 Antibody (NB100-93322)(1)

Review for Nup53 Antibody (NB100-93322) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-93322:
Filter by Applications
IF
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Nup53 NB100-93322
Enlarge
reviewed by:
Peibin Yue
IF Human 11/24/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman Panc-1 Cell Line
SpeciesHuman

Blocking

Blocking DetailsBlocked with PBS+0.1% BSA for 30 minutes at room temp.

Primary Anitbody

Dilution Ratio1:250 primary dilution in PBS-T for 1 hour at room temp.

Secondary Antibody

Secondary DescriptionAlexa Fluor Goat-anti-Rabbit 488 conjugate.
Secondary Manufacturer Cat#A-11008
Secondary Concentration1:500

Details

Detection NotesConfocal microscopy
Fixation DetailsFixed with 4% paraformaldehyde for 10 minutes at room temp. Permeabilized with PBS+0.25% Tx-100 for 10 minutes at room temp.
Wash Description3 washes in PBS-T

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nup53 Antibody (NB100-93322) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Nup53 Antibody (NB100-93322)

Discover related pathways, diseases and genes to Nup53 Antibody (NB100-93322). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for Nup53 Antibody (NB100-93322)

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Blogs on Nup53

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Contact Information

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Calculators

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Recent Reviews

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1
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Peibin Yue
11/24/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol NUP35
Entrez
Uniprot