Napsin A Antibody (TMU-Ad02)

Images

 
Western Blot: Napsin-A Antibody (TMU-Ad02) [NB110-68133] - Analysis of NAPSA expression in human lung using NB110-68133.
Immunohistochemistry-Paraffin: Napsin-A Antibody (TMU-Ad02) [NB110-68133] - Analysis of NAPSA on human lung adenocarcinoma using NB110-68133.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
TMU-Ad02
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Napsin A Antibody (TMU-Ad02) Summary

Immunogen
Synthetic peptide corresponding to the N-terminus of human NAPSA [UniProt# O96009]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NAPSA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • Immunohistochemistry 0.5-1 ug/ml
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
Application Notes
This NAPSA antibody is useful for Western blot and Immunohistochemistry on formalin-fixed, paraffin embedded sections. In WB a band can be seen around 45-50 kDa.
Control
Napsin A Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-68133 in the following applications:

Publications
Read Publications using
NB110-68133 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Napsin A Antibody (TMU-Ad02)

  • Asp 4
  • ASP4
  • Aspartyl protease 4
  • EC 3.4.23
  • EC 3.4.23.-
  • EC 3.4.23.15
  • EC 3.4.23.3
  • EC 3.4.23.5
  • KAP
  • Kdap
  • NAP1napsin-A
  • NAPA
  • NAPApronapsin A
  • NAPSA
  • napsin A aspartic peptidase
  • Napsin A
  • Napsin-1
  • SNAPA
  • TA01/TA02

Background

NAPSA (Napsin A) is an aspartic proteinase that belongs to peptidase A1 family (NAPSA, cathepsins D and E, renin, pepsin, gastricsin, and bovine chymosin) and it is primarily expressed in lung and kidney, and at a very low levels in spleen as well as in peripheral blood leukocytes. NAPSA's expression is regulated by TTF-1 which also regulates the expression of surfactant protein B. Inside the lung, NAPSA is expressed in alveolar type II pneumocytes where it colocalize with prosurfactant B protein as well as surfactant B in the lamellar bodies, and has been suggested to be involved in N-terminal/C-terminal processing of prosurfactant B protein. NAPSA is also localized in intra-alveolar macrophages, probably as a result of phagocytosis. In renal tissues, NAPSA is expressed in the proximal tubules, where it is suggested to function as a lysosomal protease involved in protein catabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Napsin A Antibody (NB110-68133)(2)

Review for Napsin A Antibody (NB110-68133) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-68133:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 11/16/2009
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman tissue
SpeciesHuman
Lot60816
CommentsThis antibody is used by immunohistochemistry.

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/1600, Primary Ab Incubation Dilution Buffer: TBS, Incubation Time: 30min, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: BIocare Polymer

Comments

CommentsThis antibody is used by immunohistochemistry.

Product General Protocols

View specific protocols for Napsin A Antibody (NB110-68133): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Napsin A Antibody (NB110-68133) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-68133AF350
Alexa Fluor 405 NB110-68133AF405
Alexa Fluor 488 NB110-68133AF488
Alexa Fluor 532 NB110-68133AF532
Alexa Fluor 594 NB110-68133AF594
Alexa Fluor 647 NB110-68133AF647
Alexa Fluor 700 NB110-68133AF700
Alexa Fluor 750 NB110-68133AF750
Biotin NB110-68133B
DyLight 350 NB110-68133UV
DyLight 405 NB110-68133V
DyLight 488 NB110-68133G
DyLight 550 NB110-68133R
DyLight 594 NB110-68133DL594
DyLight 650 NB110-68133C
DyLight 680 NB110-68133FR
DyLight 755 NB110-68133IR
FITC NB110-68133F
HRP NB110-68133H
Janelia Fluor 549 NB110-68133JF549
Janelia Fluor 646 NB110-68133JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
11/16/2009
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol NAPSA
Entrez
Uniprot