Numb Antibody (JM10-023)

Western Blot: Numb Antibody (JM10-023) [NBP2-66991] - Analysis of NUMB on A431 cells lysates using anti-NUMB antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Numb Antibody (JM10-023) [NBP2-66991] - Staining NUMB in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Numb Antibody (JM10-023) [NBP2-66991] - Analysis of paraffin-embedded rat adrenal gland tissue using anti-NUMB antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Numb Antibody (JM10-023) [NBP2-66991] - Staining NUMB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Numb Antibody (JM10-023) [NBP2-66991] - Staining NUMB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Numb Antibody (JM10-023) [NBP2-66991] - Analysis of paraffin-embedded mouse brain tissue using anti-NUMB antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Numb Antibody (JM10-023) [NBP2-66991] - Analysis of paraffin-embedded mouse kidney tissue using anti-NUMB antibody. Counter stained with hematoxylin.

Product Discontinued

Numb Antibody (JM10-023) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Numb aa 4-47 / 651. (SwissProt: P49757 Human; SwissProt: Q9QZS3 Mouse; SwissProt: Q2LC84 Rat)
• Localization: Endosome membrane, Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NUMB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Numb Antibody (JM10-023)

• h-Numb
• mnb
• m-numb
• numb (Drosophila) homolog
• numb homolog (Drosophila)
• Numb
• protein numb homolog
• Protein S171
• S171

Background

Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggestthat mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different processes. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUMB