Independent Antibodies: Western Blot: NuMA Antibody [NB100-74636] - Detection of Human NUMA on HeLa whole cell lysate using NB100-74636. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody NB100-74637.
Immunohistochemistry: NuMA Antibody [NB100-74636] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NUMA used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat ...read more
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
238.26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NB100-74636 in the following applications:
centrophilin stabilizes mitotic spindle in mitotic cells
nuclear mitotic apparatus protein 1
structural nuclear protein
The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
NuMA: The Key to Asymmetric Cell Division Nuclear Mitotic Apparatus protein (NuMA) is a cell cycle-related protein that acts as an organizer of the mitotic spindle during mitosis. It may be involved in coordinating the alignment of the mitotic spindle to the cellular polarity axis, which is a... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our NuMA Antibody and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.