Nudel Antibody - BSA Free

Western Blot: Nudel Antibody [NBP1-76677] - Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 ug/mL.

Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Jurkat cells with Nudel antibody at 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.

Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nudel Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Nudel Antibody - BSA Free (NBP1-76677) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel. The immunogen is located within the first 50 amino acids of Nudel. Amino Acid Squence: MDGEDIPDFSSLKEE
• Predicted Species: Rat (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDEL1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control Peptide:

  Nudel Peptide (NBP1-76677PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Nudel Antibody - BSA Free

• DKFZp451M0318
• EOPA
• MITAP1mitosin-associated protein MITAP1
• Mitosin-associated protein 1
• nuclear distribution protein nudE-like 1
• nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• nudE nuclear distribution gene E homolog like 1 (A. nidulans)
• nudE nuclear distribution gene E homolog like 1
• NUDELendooligopeptidase A
• NUDE-like protein
• Protein Nudel

Background

Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDEL1
• Uniprot:
  • AAF97497(Human)