Nucleolin Antibody

Images

 
Western Blot: Nucleolin Antibody [NB100-1920] - Analysis of Nucleolin in HeLa nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB100-1920] - Detection of Nucleolin (green) in Hela cells detected with a Dylight-488 conjugated secondary antibody. Nuclei are counter stained with Hoechst ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nucleolin Antibody Summary

Immunogen
A 15 residue peptide designed to the C-terminus of human Nucleolin [UniProt# P19338]
Localization
Nucleoli
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:3000-1:10000
  • Immunohistochemistry 1:5000-1:10000
  • Immunohistochemistry-Paraffin 1:5000-1:10000
  • Immunoprecipitation 1-3ug
Application Notes
This Nucleolin antibody is useful in Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot.
Publications
Read Publications using
NB100-1920 in the following applications:

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Nucleolin Antibody

  • C23
  • FLJ45706
  • FLJ59041
  • nucleolin
  • Protein C23

Background

Nucleolin (NCL, also called Protein C23) is a major phosphoprotein present in nucleolus of growing eukaryotic cells which is implicated in the synthesis as well as maturation of ribosomes. Localized mainly to nucleolus, NCL may also be found in cytoplasmic mRNP granules containing untranslated mRNAs (mRNP granule complex contains - NCL, ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs). NCL interacts with APTX, NSUN2, TERT, ERBB4, GZF1, GZF1, NVL and C1QBP, and is a part of SWAP complex (NPM1, NCL, PARP1 and SWAP70) as well as another complex containing NCL, HTATSF1/Tat-SF1, CDK9 and CCNT1, RNA polymerase II, SUPT5H. NCL may be found associated with intranucleolar chromatin and pre-ribosomal particles where it induces chromatin de-condensation via binding to histone H1 and play a key role in pre-rRNA transcription as well as ribosome assembly. NCL has also been suggested to implicate in the process of transcriptional elongation and it binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Besides being essential to rRNA processing in nucleolus, NCL has several other functions such as transcription regulation, modulation of mRNA stability and DNA replication and recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nucleolin Antibody (NB100-1920)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(2)
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(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nucleolin Antibody (NB100-1920)

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Diseases for Nucleolin Antibody (NB100-1920)

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Blogs on Nucleolin.

Nucleolin: A Multifaceted Nucleolar Phosphoprotein
Nucleolin is a ubiquitous, nonhistone nucleolar phosphoprotein of exponentially growing eukaryotic cells and is present in abundance at the dense fibrillar and granular regions of nucleolus. Intact nucleolin is the major species and represents 5% of n...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Nucleolin: To the Nucleus and Beyond!
Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expres...  Read full blog post.

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Bioinformatics

Gene Symbol NCL
Entrez
Uniprot