Nucleobindin 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] - Immunofluorescence analysis of L929 cells using [KO Validated] Nucleobindin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: Nucleobindin 1 Antibody [NBP3-38066] - Western blot analysis of various lysates using [KO Validated] Nucleobindin 1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP3-38066] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] Nucleobindin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: Nucleobindin 1 Antibody [NBP3-38066] - Western Blot analysis of lysates from wild type (WT) and Nucleobindin 1 knockout (KO) 293T cells, using [KO Validated] Nucleobindin 1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Nucleobindin 1 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human Nucleobindin 1 (NP_006175.2).
  
  Sequence:
  KNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUCB1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Nucleobindin 1 Antibody - BSA Free

• DKFZp686A15286
• FLJ40471
• NUCCALNUC
• nucleobindin 1
• nucleobindin-1

Background

Nucleobindin 1 is a major calcium binding protein of the Golgi. It may have a role in calcium homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUCB1