NTB-A/SLAMF6/CD352 Antibody (448318) [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB
• Clone: 448318
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

NTB-A/SLAMF6/CD352 Antibody (448318) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse NTB‑A/SLAMF6
  Glu31-Asn239
  Accession # Q9ET39
• Specificity: Detects mouse NTB‑A/SLAMF6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NTB-A or recombinant mouse CD84 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NTB-A/SLAMF6/CD352 Antibody (448318) [Alexa Fluor® 350]

• Activating NK receptor
• CD352 antigen
• CD352
• KALIFLJ50657
• Ly108
• NK-T-B-antigen
• NTBA receptor
• NTBA
• NTB-A
• NTB-AMGC104953
• NTBAT- and B-cell antigen
• SF2000
• SLAM family member 6
• SLAMF6

Background

NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature mouse NTB-A consists of a 209 amino acid (aa) ECD with one Ig-like V-type and one Ig-like C2-type domain, a 23 aa transmembrane segment, and an 89 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2). Within the ECD, mouse NTB-A shares 48% and 70% aa sequence identity with human and rat NTB-A, respectively. The ECD of mouse NTB-A shares 20%‑34% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. An alternatively spliced isoform diverges after the second ITSM (2). NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (3‑5). It interacts homophilically through weak associations between the Ig-V type domains (5‑7). NTB-A functions as an activating coreceptor on NK and T cells (3, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of NK cell IFN-gamma (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11). The isoform with the divergent C-terminal tail is overexpressed in B cells from lupus-prone mice (11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.