NSP 5 alpha 3 alpha Antibody [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

NSP 5 alpha 3 alpha Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: An internal synthetic peptide made to the human NSP 5 alpha 3 alpha protein sequence (between residues 1-100). [UniProt# Q5M775]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPECC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: mouse (83%), rat (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NSP 5 alpha 3 alpha Antibody [CoraFluor™ 1]

• cytospin-B
• CYTSBcytospin B
• FLJ36955
• HCMOGT-1
• NSP
• NSP5
• Nuclear structure protein 5
• sperm antigen HCMOGT 1
• Sperm antigen HCMOGT-1
• sperm antigen with calponin homology and coiled coil domains 1
• sperm antigen with calponin homology and coiled-coil domains 1cytokinesis and spindle organization B
• sperm antigen with calponin-like and coiled coil domains 1
• structure protein NSP5a3a
• structure protein NSP5a3b
• structure protein NSP5b3a
• structure protein NSP5b3b

Background

NSP 5alpha 3alpha is a novel structure protein with potential roles in ribosome biogenesis and rRNA metabolism/processing. NSP5a3a is highly expressed in certain tumor cell lines, and may serve as a good tumor marker. In these cancer lines, NSP5a3a has been shown to interact with the nucleolar apoptosis protein B23 and may be involved in a novel p73 dependent apoptosis mechanism. Therefore, NSP5a3a is expected to become an important potential target for future cancer treatment research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPECC1