NSP 5 alpha 3 alpha Antibody [FITC]

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

NSP 5 alpha 3 alpha Antibody [FITC] Summary

• Immunogen: An internal synthetic peptide made to the human NSP 5 alpha 3 alpha protein sequence (between residues 1-100). [UniProt# Q5M775]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPECC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: mouse (83%), rat (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NSP 5 alpha 3 alpha Antibody [FITC]

• cytospin-B
• CYTSBcytospin B
• FLJ36955
• HCMOGT-1
• NSP
• NSP5
• Nuclear structure protein 5
• sperm antigen HCMOGT 1
• Sperm antigen HCMOGT-1
• sperm antigen with calponin homology and coiled coil domains 1
• sperm antigen with calponin homology and coiled-coil domains 1cytokinesis and spindle organization B
• sperm antigen with calponin-like and coiled coil domains 1
• structure protein NSP5a3a
• structure protein NSP5a3b
• structure protein NSP5b3a
• structure protein NSP5b3b

Background

Nuclear structure protein 5 (NSP5) or NSP 5 alpha 3 alpha (NSP 5a3a) is a novel structural protein which has been found to be over-expressed differently in certain cancer cell lines such as: HT-29, MCF-7, Hela, HN30, Saos-2, CEM, and H23, however, it is barely detectable in normal body tissues except for testis wherein NSP 5a3a is highly expressed. NSP 5a3a interacts with cytoplasmic - nuclear proteins B23 which is known to implicate in multiple cellular processes such as mitosis, cell growth control, and apoptosis, ribosome biogenesis, and rRNA transcription/processing. NSP 5a3a also interacts with hnRNP-L which plays a role in RNA metabolism and rRNA processing. NSP proteins (NSP 5b3a, 5a3b, and 5b3b) have been implicated in several cellular activities such as intracellular trafficking, cellular/subcellular integrity, protein-protein interactions with other structural proteins as well as in protein complex stabilization and scaffolding thus facilitating homo or hetero dimerization of protein complexes. Over-expression of NSP 5a3a has been shown to induce apoptosis in head and neck carcinoma cell lines in particular in HN30 being p53 independent yet involving a novel p73 dependent mechanism wherein NSP 5a3a induces apoptosis through p73-DAXX and TRAF2-TRADD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPECC1