BCAR3 Antibody

Images

 
Western Blot: BCAR3 Antibody [NB100-87003] - Detection of Human BCAR3 on HeLa whole cell lysate using NB100-87003. BCAR3 was also immunoprecipitated by another rabbit anti-BCAR3 antibody.
Proximity Ligation Assay: BCAR3 Antibody [NB100-87003] - Secondary-conjugate Duolink II PLA in Hela cells. goat-anti human FUS (NBP2-14860) and rabbit anti-human BCAR3 (NB100-87003). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: BCAR3 Antibody [NB100-87003] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RCC1 (NB110-40590) and rabbit anti-human BCAR3 (NB100-87003). Image merged from DAPI (2ms) and ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

BCAR3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human breast cancer anti-estrogen resistance 3 using the numbering given in entry NP_003558.1 (GeneID 8412).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCAR3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200-1:4000
Application Notes
Use in Rat reported in customer review.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
BCAR3 Lysate (NBL1-07936)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-87003 in the following applications:

Publications
Read Publication using NB100-87003.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCAR3 Antibody

  • breast cancer estrogen resistance 3
  • dJ1033H22.2 (breast cancer estrogen resistance 3)
  • KIAA0554
  • Novel SH2-containing protein 2
  • NSP2SH2D3Bbreast cancer estrogen resistance protein 3
  • SH2 domain-containing protein 3B

Background

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BCAR3 Antibody (NB100-87003)(1)

Reviews for BCAR3 Antibody (NB100-87003) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NB100-87003:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot BCAR3 NB100-87003
Enlarge
reviewed by:
Anonymous
WB Human 07/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 total lysate,GST-pull down assay
SpeciesHuman
LotA1
Western Blot BCAR3 NB100-87003
Enlarge
reviewed by:
Anonymous
WB Human 02/09/2010
View

Summary

ApplicationWestern Blot
Sample Testedhuman pancreatic cancer cell lines and human pancreatic primary tumors, Sample Amount: 30 to 40 ug
SpeciesHuman
CommentsBlotted lysates from various human pancreatic cancer cell lines. Block well with 5% milk in TBST and incubate membrane in primary antibody O/N at 4 degrees C diluted 1:1000. Was very specific as I used it in RNAi knockdown studies to prove knockdown of BCAR3 protein. Detection Method: Millipore sensitive ECL, Exposure Time: 15 seconds, 30 seconds & 1 minute, Positive Control: Mia-Paca-2 human pancreatic cancer cell line, Negative Control: Capan-2 human pancreatic cancer cell line, Specific Bands: 90 kDa, Non-Specific Bands: I forget, there were some but not too many.

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Cell Signaling anti-rabbit IgG-HRP

Details

Detection NotesPlease see Comments

Comments

CommentsBlotted lysates from various human pancreatic cancer cell lines. Block well with 5% milk in TBST and incubate membrane in primary antibody O/N at 4 degrees C diluted 1:1000. Was very specific as I used it in RNAi knockdown studies to prove knockdown of BCAR3 protein. Detection Method: Millipore sensitive ECL, Exposure Time: 15 seconds, 30 seconds & 1 minute, Positive Control: Mia-Paca-2 human pancreatic cancer cell line, Negative Control: Capan-2 human pancreatic cancer cell line, Specific Bands: 90 kDa, Non-Specific Bands: I forget, there were some but not too many.

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FAQs for BCAR3 Antibody (NB100-87003) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Anonymous
07/18/2017
Application: WB
Species: Human

Anonymous
02/09/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol BCAR3
Entrez
Uniprot