NSFL1C Antibody - Azide and BSA Free

Western Blot: NSFL1C Antibody [H00055968-B01P] - Analysis of NSFL1C expression in human placenta.

Immunocytochemistry/Immunofluorescence: NSFL1C Antibody [H00055968-B01P] - Analysis of purified antibody to NSFL1C on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: NSFL1C Antibody [H00055968-B01P] - Analysis of NSFL1C expression in transfected 293T cell line by NSFL1C polyclonal antibody. Lane 1: NSFL1C transfected lysate(40.7 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NSFL1C Antibody - Azide and BSA Free Summary

• Immunogen: NSFL1C (NP_057227, 1 a.a. - 370 a.a.) full-length human protein. MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT
• Specificity: NSFL1C - NSFL1 (p97) cofactor (p47),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: NSFL1C
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NSFL1C Antibody - Azide and BSA Free

• dJ776F14.1
• NSFL1 (p97) cofactor (p47)
• NSFL1 cofactor p47
• p47
• p97 cofactor p47
• SHP1 homolog
• UBX domain protein 2C
• UBX domain-containing protein 2C
• UBX1
• UBXD10
• UBXN2CMGC3347

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NSFL1C Antibody (H00055968-B01P)(2)

Publications using H00055968-B01P

• Sarkar B, Nishikata I, Nakahata S et al. Degradation of p47 by autophagy contributes to CADM1 overexpression in ATLL cells through the activation of NF-kappa B. Sci Rep. 2019-03-05 [PMID: 30837480]
• Shibata Y, Oyama M, Kozuka-Hata H et al. p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO. Nat Commun. 2012-01-01 [PMID: 22990857] (Human)

Bioinformatics

• Gene Symbol: NSFL1C
• Entrez:
  • 55968(Human)
• Uniprot:
  • NP_057227(Human)