Nrf2 [p Ser40] Antibody (SU0334)

Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Western blot analysis of Nrf2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Flow Cytometry: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Analysis of RIP3 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Rat kidney tissue using anti-RIP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Analysis of RIP3 on HT-29 cell lysates with Rabbit anti-RIP3 antibody at 1/1,000 dilution.Lysates/proteins at 10 ug/Lane.Predicted band size: 57 kDaObserved band size: 50 kDaExposure time: 2 minutes;12% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: SU0334
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Nrf2 [p Ser40] Antibody (SU0334) Summary

• Description: Novus Biologicals Rabbit Nrf2 [p Ser40] Antibody (SU0334) (NBP2-67465) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. Anti-Nrf2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser40 of Human Nrf2 aa 20-69 / 605. (SwissProt: Q16236 Human)
• Modification: p Ser40
• Localization: Cytoplasm, Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFE2L2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2,000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Nrf2 [p Ser40] Antibody (SU0334)

• HEBP1
• NFE2L2
• NF-E2-related factor 2
• NFE2-related factor 2
• NRF2 erythroid derived 2, like 2
• Nrf2
• nuclear factor (erythroid-derived 2)-like 2
• nuclear factor erythroid 2-related factor 2
• nuclear factor erythroid-derived 2-like 2

Background

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nrf2 Antibody (NBP2-67465)(4)

Publications using NBP2-67465

• Schwertheim S, Alhardan M, Manka PP et al. Higher pNRF2, SOCS3, IRF3, and RIG1 Tissue Protein Expression in NASH Patients versus NAFL Patients: pNRF2 Expression Is Concomitantly Associated with Elevated Fasting Glucose Levels Journal of Personalized Medicine 2023-07-18 [PMID: 37511764]
• Park JW, Oh JH, Hwang D et al. 3,4,5-Trihydroxycinnamic acid exerts anti inflammatory effects on TNF alpha/IFN gamma stimulated HaCaT cells Molecular medicine reports 2021-07-01 [PMID: 33982762] (WB, Human)
• De Blasio A, Di Fiore R, Pratelli G et al. A loop involving NRF2, miR-29b-1-5p and AKT, regulates cell fate of MDA-MB-231 triple-negative breast cancer cells J. Cell. Physiol. 2019-07-10 [PMID: 31313842]
• Yang J, Choi M, Shin H et al. Phyllostachys nigra Variety Henosis, a Domestic Bamboo Species, Protects PC12 Cells from Oxidative Stress-mediated Cell Injury through Nrf2 Activation Biotechnology and Bioprocess Engineering 2022-06-21

Bioinformatics

• Gene Symbol: NFE2L2