NPY2R Antibody - BSA Free

Western Blot: NPY2R Antibody [NB110-59978] - analysis of NPY2R in rat hypothalamus lysate using anti-NPY2R antibody. Image from verified customer review.

Immunohistochemistry: NPY2R Antibody [NB110-59978] - Image of NPY Y2 receptor immunoreactivity in mouse dorsal root ganglion of wild type (A) and NPY Y2 receptor Knockout (B) animals. Note that unspecific staining is observed in thick, possibly myelinated fibers.

Product Discontinued

NPY2R Antibody - BSA Free Summary

• Immunogen: (C)TDSFSEATNV. Cys added for conjugation to KLH
• Specificity: NPY Y2 Receptor
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPY2R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
  • Western Blot
• Application Notes: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in IHC-Fr reported in scientific literature (PMID:35902640).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.28 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NPY2R Antibody - BSA Free

• neuropeptide Y receptor type 2
• neuropeptide Y receptor Y2
• NPY2R
• NPY2-R
• NPY-Y2 receptor
• Y2 Receptor

Background

Neuropeptide Y (NPY) receptors are members of a G protein coupled receptor superfamily and they mediate a variety of physiological responses including feeding and vasoconstriction. The endogenous ligands of NPY receptors are a family of structurally related peptides, including NPY, peptide YY (PYY), and pancreatic polypeptide (PP). Increased activity of NPY and its receptors, Y1R, Y2R, and Y5R has been found in the brain in many forms of experimental obesity. Furthermore, several association studies have supported the association between NPY2R and obesity. This NPY Y2 antibody has been extensively characterized in the spinal cord, brain and distal colon.This NPY Y2 antibody has been extensively characterized at the spinal level and is currently being characerized in brain. The antibody has been tested in both rat and mouse and appears to work better on mose tissue, although positive staining is seen in rat. For full characterizarion in dorsal root ganglion and spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPY2R Antibody (NB110-59978)(2)

Publications using NB110-59978

• Chakroborty D, Goswami S, Fan H et al. Neuropeptide Y, a paracrine factor secreted by cancer cells, is an independent regulator of angiogenesis in colon cancer British journal of cancer 2022-07-28 [PMID: 35902640] (WB, IHC-P, IHC-Fr)
• Yarosh HL, Angulo JA. Modulation of methamphetamine-induced nitric oxide production by neuropeptide Y in the murine striatum Brain Res 2012-09-13 [PMID: 22982589] (ICC/IF, IF/IHC, Mouse)

Review for NPY2R Antibody (NB110-59978) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 04/23/2015

Summary

• Application: Western Blot
• Species: Rat

Bioinformatics

• Gene Symbol: NPY2R
• Entrez:
  • 66024(Rat)