NPY5R Antibody

Images

 
Western Blot: NPY5R Antibody [NB100-1538] - Staining of Human Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NPY5R Antibody [NB100-1538] - Staining of Human Intestine. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Rt, Ha, Mu, CaSpecies Glossary
Applications WB, IHC, IHC-P, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NPY5R Antibody Summary

Immunogen
Peptide with sequence C-GLSNKENRLEEN corresponding to internal region according to NP_006165.1.
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPY5R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 6 ug/mL
  • Immunohistochemistry-Paraffin 4 - 6 ug/mL
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
This NPY5R Antibody is validated for IP from a verified customer review. WB: Approx. 50 kDa band observed in human brain, breast and duodenum lysates (calculated MW of 50.7 kDa band according to NP_006165.1). IHC-P: Human intestine shows staining of ganglion cells.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB100-1538 in the following applications:

  • WB
    3 publications

Reactivity Notes

Rat reactivity reported in (PMID: 23179670).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NPY5R Antibody

  • neuropeptide Y receptor type 5
  • neuropeptide Y receptor Y5
  • NPY5R
  • NPYR5NPY5-R
  • NPY-Y5 receptor
  • NPYY5-R
  • Y5 receptor

Background

NPY5R is a receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NPY5R Antibody (NB100-1538)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NPY5R Antibody (NB100-1538). (Showing 1 - 1 of 1 FAQs).

  1. Why dose your Y5R antibodies produces 2 different molecular weight products in human tissues or human cell lysates? NB100-1538 ab probes to 50KDa protein while the rabbit Y5R ab probes to 40-42KDa protein?
    • NPY5R is a glycosylated protein. This can cause it to run at varying molecular weights depending on the cell line and Western blot conditions. The 48 kDa band represents the protein in it's unglycosylated form whereas the higher molecular weights above 50 kDa are from blots generated in conditions when the protein is in its glycosylated form.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NPY5R
Entrez
Uniprot