NPY1R Antibody (556153)

MCF‑7 human breast cancer cell line (Korner, M and J.C. Reubi (2007) Peptides 28:419) was stained with Human NPY1R Monoclonal Antibody (Catalog # MAB6400, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 556153
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NPY1R Antibody (556153) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human NPY1R
  Met1-Ile384
  Accession # P25929
• Specificity: Detects human NPY1R in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPY1R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NPY1R Antibody (556153)

• neuropeptide Y receptor type 1
• neuropeptide Y receptor Y1
• NPY1R
• NPY1-R
• NPYR
• NPYY1

Background

Neuropeptide Y receptor type 1 (NPY1R) is a 44 kDa (unglycosylated) member of the G protein coupled receptor 1 family. Human NPY1R is 384 amino acids (aa) in length and contains seven transmembrane regions and three potential sites for N-linked glycosylation. In addition, aa 338 is an S-palmitoyl cysteine lipid-binding site and aa 368 is a phosphoserine. Human NPY1R shares 94% and 93% aa sequence identity with mouse and rat NPY1R, respectively. Functionally, NPY1R is a receptor for neuropeptide Y and peptide YY.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPY1R Antibody (MAB6400)(1)

Publication using MAB6400

• Chewei Anderson Chang, Jayu Jen, Shaowen Jiang, Azin Sayad, Arvind Singh Mer, Kevin R. Brown et al. Ontogeny and Vulnerabilities of Drug-Tolerant Persisters in HER2+ Breast Cancer Cancer Discovery 2022-04-01 [PMID: 34911733]

Bioinformatics

• Gene Symbol: NPY1R
• Uniprot:
  • P25929()