NOXO1 Antibody

Images

 
Immunohistochemistry-Paraffin: NOXO1 Antibody [NB300-950] - IHC analysis of NOXO1 in formalin-fixed, paraffin-embedded human kidneyusing this antibody at 10 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NOXO1 Antibody Summary

Immunogen
Peptide with sequence RGCVDSVPHPTTEQ corresponding to C-Terminus according to NP_653204.1, NP_751907.1, NP_751907.1.
Specificity
This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOXO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 10 - 20 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave no signal but low background in Hela and human heart lysates at up to 1 ug/ml. IHC-P: Human kidney cortex shows cytoplasmic stain in epithelial cells of some PCT.
Control
NOXO1 Overexpression Lysate
Publications
Read Publications using
NB300-950 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28824611).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NOXO1 Antibody

  • MGC20258
  • NADPH oxidase organizer 1
  • Nox organizer 1
  • Nox-organizing protein 1
  • P41NOX
  • P41NOXA
  • P41NOXB
  • P41NOXC
  • regulatory protein P41NOX
  • SH3 and PX domain-containing protein 5
  • SH3PXD5NADPH oxidase regulatory protein
  • SNX28

Background

NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Co-transfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5-P2, PtdIns 5-P and PtdIns 4-P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 co-localizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31546
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-41012
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
NB100-790
Species: Hu, Mu, Po, Ba
Applications: WB, Flow, IHC, IHC-P, IP, PEP-ELISA, IF
NBP2-13677
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF5758
Species: Hu
Applications: WB, IHC, ICC
MAB1904
Species: Hu
Applications: WB, IHC, ICC
NBP1-89163
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-40974
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IP
NB100-1343
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-84254
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13754
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-98673
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-33736
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82542
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-80467
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-83139
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22377
Species: Hu
Applications: WB, IHC, IHC-P

Publications for NOXO1 Antibody (NB300-950)(4)

Reviews for NOXO1 Antibody (NB300-950) (0)

There are no reviews for NOXO1 Antibody (NB300-950). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NOXO1 Antibody (NB300-950) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional NOXO1 Products

Bioinformatics Tool for NOXO1 Antibody (NB300-950)

Discover related pathways, diseases and genes to NOXO1 Antibody (NB300-950). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NOXO1 Antibody (NB300-950)

Discover more about diseases related to NOXO1 Antibody (NB300-950).
 

Pathways for NOXO1 Antibody (NB300-950)

View related products by pathway.

PTMs for NOXO1 Antibody (NB300-950)

Learn more about PTMs related to NOXO1 Antibody (NB300-950).
 

Research Areas for NOXO1 Antibody (NB300-950)

Find related products by research area.

Blogs on NOXO1

There are no specific blogs for NOXO1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NOXO1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NOXO1
Entrez
Uniprot