NOXO1 Antibody

Immunohistochemistry-Paraffin: NOXO1 Antibody [NB300-950] - IHC analysis of NOXO1 in formalin-fixed, paraffin-embedded human kidneyusing this antibody at 10 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NOXO1 Antibody Summary

• Description: Novus Biologicals Goat NOXO1 Antibody (NB300-950) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-NOXO1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RGCVDSVPHPTTEQ corresponding to C-Terminus according to NP_653204.1, NP_751907.1, NP_751907.1.
• Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NOXO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 20 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave no signal but low background in Hela and human heart lysates at up to 1 ug/ml. IHC-P: Human kidney cortex shows cytoplasmic stain in epithelial cells of some PCT.
• Control:

  NOXO1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28824611).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NOXO1 Antibody

• MGC20258
• NADPH oxidase organizer 1
• Nox organizer 1
• Nox-organizing protein 1
• P41NOX
• P41NOXA
• P41NOXB
• P41NOXC
• regulatory protein P41NOX
• SH3 and PX domain-containing protein 5
• SH3PXD5NADPH oxidase regulatory protein
• SNX28

Background

NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Co-transfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5-P2, PtdIns 5-P and PtdIns 4-P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 co-localizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NOXO1 Antibody (NB300-950)(4)

Publications using NB300-950

• Radbruch H, Mothes R, Bremer D et al. Analyzing Nicotinamide Adenine Dinucleotide Phosphate Oxidase Activation in Aging and Vascular Amyloid Pathology Front Immunol 2017-08-21 [PMID: 28824611] (IF/IHC, Mouse)
• Chung YW, Kim HK, Kim IY et al. Dual function of protein kinase C (PKC) in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced manganese superoxide dismutase (MnSOD) expression: activation of CREB and FOXO3a by PKC-alpha phosphorylation and by PKC-mediated inactivation of Akt, respectively. J Biol Chem. 2011-08-26 [PMID: 21705328] (WB)
  
  Details:
  NOXO1 (IMG-3062). WB: A549 cell line treated with NOXO1 siRNA, Fig 1E. Note: NOXO1 antibody was siRNA validated by WB in A549 cell line, Fig 1E.
• Wu S, Gao J, Ohlemeyer C et al. Activation of AP-1 through reactive oxygen species by angiotensin II in rat cardiomyocytes. Free Radic Biol Med. 2005-12-15 [PMID: 16298685]
• Banfi B, Clark RA, Steger K, Krause KH. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J Biol Chem 2003-02-07 [PMID: 12473664]

Bioinformatics

• Gene Symbol: NOXO1
• Entrez:
  • 124056(Human)
• Uniprot:
  • NP_653204(Human)