Notch-1 Antibody (527425) [Phycoerythrin]

U2OS human osteosarcoma cell line was stained with Mouse Anti-Human Notch-1 PE-conjugated Monoclonal Antibody (Catalog # FAB5317P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 527425
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Notch-1 Antibody (527425) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Notch-1
  Ala19-Gln526
  Accession # P46531
• Specificity: Detects human Notch-1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOTCH1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Notch-1 Antibody (527425) [Phycoerythrin]

• EC 2.1.2.11
• EC 3.4.21.68
• hN1
• Notch (Drosophila) homolog 1 (translocation-associated)
• notch 1Notch homolog 1, translocation-associated (Drosophila)
• Notch homolog 1, translocation-associated
• Notch1
• Notch-1
• TAN1
• TAN1neurogenic locus notch homolog protein 1
• Translocation-associated notch protein TAN-1

Background

Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1‑3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2‑4). Notch can specify binary cell fates; for example, promoting T‑ over B‑cell development from a common precursor (2). More than 50% of human T-lineage Acute Lymphoblastic Leukemia (T‑ALL) have activating mutations of Notch1 (1, 5). Human Notch-1 is synthesized as a 2556 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1718 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11^th and 12^th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (6). O-fucosylation by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647 aa ligand-binding extracellular region with the 891 aa transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM-17) and the presenilin-dependent gamma -Secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Human Notch-1 ECD aa 19 - 526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat, 89% with canine, and 79% with chicken Notch-1. This region also exhibits 60% aa identity with human Notch-2 and Notch-3.

1. Ellisen, L. W. et al. (1991) Cell 66:649.
2. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
4. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823.
5. Weng, A. P. et al. (2004) Science 306:269.
6. Hambleton, S. et al. (2004) Structure 12:2173.
7. Yang, L. et al. (2005) Mol. Biol. Cell 16:927.
8. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
9. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
10. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Notch-1 Antibody (FAB5317P)(6)

Publications using FAB5317P

• Matteo Marchesini, Andrea Gherli, Anna Montanaro, Laura Patrizi, Claudia Sorrentino, Luca Pagliaro et al. Blockade of Oncogenic NOTCH1 with the SERCA Inhibitor CAD204520 in T Cell Acute Lymphoblastic Leukemia Cell Chemical Biology 2020-06-01 [PMID: 32386594]
• YV Teo, N Rattanavir, N Olova, A Salzano, A Quintanill, N Tarrats, C Kiourtis, M Müller, AR Green, PD Adams, JC Acosta, TG Bird, K Kirschner, N Neretti, T Chandra Notch Signaling Mediates Secondary Senescence Cell Rep, 2019-04-23;27(4):997-1007.e5. 2019-04-23 [PMID: 31018144] (Flow Cytometry, Human)
• A Al-Attar, Y Alimova, S Kirakodu, A Kozal, MJ Novak, AJ Stromberg, L Orraca, J Gonzalez-M, M Martinez, JL Ebersole, OA Gonzalez Activation of Notch-1 in oral epithelial cells by P. gingivalis triggers the expression of the antimicrobial protein PLA2-IIA Mucosal Immunol, 2018-03-07;11(4):1047-1059. 2018-03-07 [PMID: 29515164] (Flow Cytometry, Human)
• S Baldoni, B Del Papa, E Dorillo, P Aureli, F De Falco, C Rompietti, D Sorcini, E Varasano, D Cecchini, T Zei, A Di Tommaso, E Rosati, G Alexe, G Roti, K Stegmaier, M Di Ianni, F Falzetti, P Sportolett Bepridil exhibits anti-leukemic activity associated with NOTCH1 pathway inhibition in chronic lymphocytic leukemia Int. J. Cancer, 2018-03-23;0(0):. 2018-03-23 [PMID: 29508386] (Flow Cytometry, Human)
• Giovanni Roti, Jun Qi, Samuel Kitara, Marta Sanchez-Martin, Amy Saur Conway, Anthony C. Varca et al. Leukemia-specific delivery of mutant NOTCH1 targeted therapy Journal of Experimental Medicine 2018-01-02 [PMID: 29158376]
• Piggott K, Deng J, Warrington K, Younge B, Kubo JT, Desai M, Goronzy JJ, Weyand CM Blocking the NOTCH pathway inhibits vascular inflammation in large-vessel vasculitis. Circulation, 2011-01-10;123(3):309-18. 2011-01-10 [PMID: 21220737] (Flow Cytometry, Human)

FAQs for Notch-1 Antibody (FAB5317P). (Showing 1 - of FAQs).

Bioinformatics

• Gene Symbol: NOTCH1
• Uniprot:
  • P46531()