non-muscle Myosin IIA Antibody - BSA Free

Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human smooth muscle shows moderate cytoplasmic positivity in muscle fibres and endothelial cells.

Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in glomeruli.

Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human endometrium shows moderate membranous positivity in glandular cells.

Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human testis shows moderate cytoplasmic/membranous positivity in Leydig cells.

Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Immunohistochemistry analysis in human endometrium and skeletal muscle tissues using NBP2-62644 antibody. Corresponding MYH9 RNA-seq data are presented for the same tissues.

Staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & actin filaments.

Staining of human endometrium, kidney, skeletal muscle and testis using NBP2-62644 (A) shows similar protein distribution across tissues to independent antibody NBP1-85244 (B).

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

non-muscle Myosin IIA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit non-muscle Myosin IIA Antibody - BSA Free (NBP2-62644) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH9
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1.0 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  non-muscle Myosin IIA Recombinant Protein Antigen (NBP2-62644PEP)

Reactivity Notes

Mouse (88%), Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for non-muscle Myosin IIA Antibody - BSA Free

• Cellular myosin heavy chain, type A
• DFNA17
• EPSTS
• FTNSNMMHC-IIA
• MGC104539
• MHANMMHC II-a
• MYH9 variant protein
• MYH9
• Myosin heavy chain 9
• Myosin heavy chain, non-muscle IIa
• myosin, heavy chain 9, non-muscle
• myosin, heavy polypeptide 9, non-muscle
• myosin-9
• NMHC-II-A
• NMMHC II-a
• NMMHCA
• NMMHC-A
• NMMHC-IIA
• Non-muscle myosin heavy chain A
• Non-muscle myosin heavy chain IIa
• nonmuscle myosin heavy chain II-A
• non-muscle myosin heavy polypeptide 9
• Non-muscle Myosin IIA

Background

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH9