NRAMP1/SLC11A1 Antibody (910825)

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NRAMP1/SLC11A1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for NRAMP1/SLC11A1 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with either (A) human NRAMP1/SLC11A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 910825
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NRAMP1/SLC11A1 Antibody (910825) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human NRAMP1/SLC11A1
  Met1-Gly550
  Accession # NP_000569
• Specificity: Detects HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC11A1
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NRAMP1/SLC11A1 Antibody (910825)

• natural resistance-associated macrophage protein 1
• LSH
• member 1
• NRAMP 1
• NRAMP1
• NRAMP1solute carrier family 11 (sodium/phosphate symporters), member 1
• NRAMPnatural resistance-associated macrophage protein 1
• SLC11A1
• solute carrier family 11 (proton-coupled divalent metal ion transporters)

Background

NRAMP1/SLC11A1 (Natural Resistance-Associated Macrophage Protein 1/Solute Carrier family 11, member 1) is a member of the solute carrier family of multi-pass membrane proteins. It functions as a proton-coupled divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in SLC11A1 have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn’s disease. Alternatively spliced variants that encode different protein isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC11A1
• Uniprot:
  • NP_000569()