NOM1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: NOM1 Antibody [NBP2-47352] - Staining of human cell line MCF7 shows localization to nucleoli. Antibody staining is shown in green.

Immunohistochemistry: NOM1 Antibody [NBP2-47352] - Staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NOM1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NOM1 Antibody - BSA Free (NBP2-47352) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ITDKSLCGSGEKYIPPHVRQAEETVDFKKKEELERLKKHVKGLLNRLSEPNMASISGQLEELYMAHSRKDMNDTLTSALMGACVTASAMPSRLMMEHVLLVSILHHTVGIEVGAHFLEAVVRKFDAIYKYGSE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOM1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NOM1 Protein (NBP2-47352PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NOM1 Antibody - BSA Free

• C7orf3
• chromosome 7 open reading frame 3
• FLJ16401
• nucleolar MIF4G domain-containing protein 1
• nucleolar protein with MIF4G domain 1
• SGD1 homolog
• SGD1

Background

Proteins that contain MIF4G (middle of eIF4G (MIM 600495)) and/or MA3 domains, such as NOM1, function in proteintranslation. These domains include binding sites for members of the EIF4A family of ATP-dependent DEAD box RNAhelicases (see EIF4A1; MIM 602641) (Simmons et al., 2005 (PubMed 15715967)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOM1