Protein Phosphatase 1 beta Antibody

Images

 
Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP1-32618] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: PPP1CB protein stained by PPP1CB antibody iluted at 1:100. ...read more
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Mouse hind brain dilution: 1:500.
Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - (30ug whole cell lysate) A: 293T- B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Mouse lung. Paraffin-embedded mouse lung. Dilution: 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Protein Phosphatase 1 beta Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PPP1CB. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1CB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (100%), Expected cross reactivity based on sequence homology: Xenopus Tropicalis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protein Phosphatase 1 beta Antibody

  • EC 3.1.3.16
  • EC 3.1.3.53
  • MGC3672
  • PP1beta
  • PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform
  • PPP1CD
  • protein phosphatase 1, catalytic subunit, beta isoform
  • protein phosphatase 1, catalytic subunit, beta isozyme
  • protein phosphatase 1-beta
  • protein phosphatase 1-delta
  • serine/threonine protein phosphatase PP1-beta catalytic subunit
  • serine/threonine-protein phosphatase PP1-beta catalytic subunit

Background

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31348
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NLS1071
Species: Hu
Applications: IHC-P
MAB3000
Species: Hu, Mu, Rt
Applications: WB
AF6465
Species: Hu, Rt
Applications: WB, ICC
NBP1-85747
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P, IP
AF1103
Species: Hu
Applications: WB, IHC, ICC
NB120-2928
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB110-38889
Species: Hu
Applications: WB, IP
NB110-61646
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-27202
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Op, Other, Pm
Applications: WB, Simple Western
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP1-92218
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-44092
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-56745
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IP
NB100-598
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-02272
Species: Hu, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-07799
Species: Hu
NBP1-85789
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Protein Phosphatase 1 beta Antibody (NBP1-32618) (0)

There are no publications for Protein Phosphatase 1 beta Antibody (NBP1-32618).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protein Phosphatase 1 beta Antibody (NBP1-32618) (0)

There are no reviews for Protein Phosphatase 1 beta Antibody (NBP1-32618). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Protein Phosphatase 1 beta Antibody (NBP1-32618) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Protein Phosphatase 1 beta Products

Bioinformatics Tool for Protein Phosphatase 1 beta Antibody (NBP1-32618)

Discover related pathways, diseases and genes to Protein Phosphatase 1 beta Antibody (NBP1-32618). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Protein Phosphatase 1 beta Antibody (NBP1-32618)

Discover more about diseases related to Protein Phosphatase 1 beta Antibody (NBP1-32618).
 

Pathways for Protein Phosphatase 1 beta Antibody (NBP1-32618)

View related products by pathway.

PTMs for Protein Phosphatase 1 beta Antibody (NBP1-32618)

Learn more about PTMs related to Protein Phosphatase 1 beta Antibody (NBP1-32618).
 

Research Areas for Protein Phosphatase 1 beta Antibody (NBP1-32618)

Find related products by research area.

Blogs on Protein Phosphatase 1 beta.

Myc-tag: The "Monkey Wrench" of Proteomic Tools
c-Myc is a well-characterized transcription factor encoded by the c-Myc gene on human chromosome 8q24. This cellular proto-oncogene, also known as p62, is commonly activated in a variety of tumor cells and plays a crucial role in cellular proliferatio...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Protein Phosphatase 1 beta Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PPP1CB