PNMA3 Antibody (OTI1C3)

Western Blot: PNMA3 Antibody (1C3) [NBP2-03547] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMA3.

Immunohistochemistry-Paraffin: PNMA3 Antibody (1C3) [NBP2-03547] Staining of paraffin-embedded Human Kidney tissue using anti-PNMA3 mouse monoclonal antibody.

Flow Cytometry: PNMA3 Antibody (1C3) [NBP2-03547] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PNMA3 antibody, and then analyzed by flow cytometry.

Flow Cytometry: PNMA3 Antibody (1C3) [NBP2-03547] - Analysis of Hela cells, using anti-PNMA3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: PNMA3 Antibody (1C3) [NBP2-03547] - Analysis of Jurkat cells, using anti-PNMA3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: OTI1C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.65 mg/ml

PNMA3 Antibody (OTI1C3) Summary

• Description: Novus Biologicals Mouse PNMA3 Antibody (OTI1C3) (NBP2-03547) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PNMA3 (NP_037496) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PNMA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500
• Theoretical MW: 52.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.65 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PNMA3 Antibody (OTI1C3)

• MA3MA5
• MGC132756
• MGC132758
• paraneoplastic antigen MA3
• paraneoplastic cancer-testis-brain antigen

Background

The PNMA3 gene is a member of the paraneoplastic antigen MA (PNMA) gene family, whose protein products share homology with retroviral Gag proteins. They are highly expressed in the brain and also in a range of tumors associated with serious neurological phenot

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNMA3
• Entrez:
  • 37496(Human)