NOD2 Antibody

Images

 
Western Blot: NOD2 Antibody [NB500-253] - Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate using NB 500-253. ECL detection in 15 seconds.
Immunocytochemistry/ Immunofluorescence: NOD2 Antibody [NB500-253] - NOD2 antibody was tested in HT-29 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: NOD2 Antibody [NB500-253] - Analysis of FFPE human skin using NOD2 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.87 mg/ml

NOD2 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human NOD2 (between residues 1000-1040). [UniProt# Q9HC29]
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation
Application Notes
This NOD2 antibody has been tested for Western blot on NOD2 transfected 293T lysates where a band is seen at ~110 kDa. We did not see positive results probing endogenous protein using HT29 lysates. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunoprecipitation reported in scientific literature (PMID:30709874).
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NOD2 Peptide (NB500-253PEP)
Publications
Read Publications using
NB500-253 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.87 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NOD2 Antibody

  • BLAU
  • CARD15caspase recruitment domain protein 15
  • caspase recruitment domain family, member 15
  • Caspase recruitment domain-containing protein 15
  • CDACUG
  • CLR16.3
  • IBD1NLR family, CARD domain containing 2
  • Inflammatory bowel disease protein 1
  • NLRC2
  • NOD-like receptor C2
  • nucleotide-binding oligomerization domain 2
  • nucleotide-binding oligomerization domain containing 2
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
  • nucleotide-binding oligomerization domain-containing protein 2
  • PSORAS1NOD2B

Background

The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NOD2 Antibody (NB500-253)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-P
(1)
IP
(1)
WB
(2)
All Applications
Filter By Species
Human
(4)
All Species

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOD2 Antibody (NB500-253). (Showing 1 - 1 of 1 FAQs).

  1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
    • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 (http://www.novusbio.com/NB100-524). This antibody has been validated for use in WB, IHC-Paraffin and IP.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-253AF350
Alexa Fluor 405 NB500-253AF405
Alexa Fluor 488 NB500-253AF488
Alexa Fluor 532 NB500-253AF532
Alexa Fluor 594 NB500-253AF594
Alexa Fluor 647 NB500-253AF647
Alexa Fluor 700 NB500-253AF700
Alexa Fluor 750 NB500-253AF750
Biotin NB500-253B
DyLight 405 NB500-253V
DyLight 488 NB500-253G
DyLight 550 NB500-253R
DyLight 594 NB500-253DL594
DyLight 650 NB500-253C
DyLight 680 NB500-253FR
DyLight 755 NB500-253IR
FITC NB500-253F
HRP NB500-253H
Janelia Fluor 549 NB500-253JF549
Janelia Fluor 646 NB500-253JF646

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Blogs on NOD2.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

NOD2 - inflammatory signaling and NFkB activation
Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) is an intracellular pattern recognition receptor (PRR) that plays an important role in recognizing bacterial pathogens and initiating an immune response. As a PRR, NOD2 recogniz...  Read full blog post.

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Bioinformatics

Gene Symbol NOD2