Western Blot: NOD2 Antibody [NB500-253] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 500-253. ECL detection in 15 seconds.
Immunocytochemistry/ Immunofluorescence: NOD2 Antibody [NB500-253] - NOD2 antibody was tested in HT-29 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
This NOD2 antibody has been tested for Western blot on NOD2 transfected 293T lysates where a band is seen at ~110 kDa. We did not see positive results probing endogenous protein using HT29 lysates.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
nucleotide-binding oligomerization domain-containing protein 2
The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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