NOD2 Antibody


Western Blot: NOD2 Antibody [NB500-253] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 500-253. ECL detection in 15 seconds.
Immunocytochemistry/ Immunofluorescence: NOD2 Antibody [NB500-253] - NOD2 antibody was tested in HT-29 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
0.87 mg/ml

NOD2 Antibody Summary

A synthetic peptide made to the C-terminal region of human NOD2 (between residues 1000-1040). [UniProt# Q9HC29]
Predicted Species
Primate (100%)
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
0.87 mg/ml
Immunogen affinity purified


  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
Application Notes
This NOD2 antibody has been tested for Western blot on NOD2 transfected 293T lysates where a band is seen at ~110 kDa. We did not see positive results probing endogenous protein using HT29 lysates.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa
Control Peptide
NOD2 Peptide (NB500-253PEP)
Read Publications using
NB500-253 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Human. Predicted to react with monkey based on 100% sequence homology.

Alternate Names for NOD2 Antibody

  • BLAU
  • CARD15caspase recruitment domain protein 15
  • caspase recruitment domain family, member 15
  • Caspase recruitment domain-containing protein 15
  • CLR16.3
  • IBD1NLR family, CARD domain containing 2
  • Inflammatory bowel disease protein 1
  • NLRC2
  • NOD-like receptor C2
  • nucleotide-binding oligomerization domain 2
  • nucleotide-binding oligomerization domain containing 2
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
  • nucleotide-binding oligomerization domain-containing protein 2


The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: WB, IHC
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, IF
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: Flow, Func, In vitro, In vivo, Flow-CS
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, Flow, AgAct
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for NOD2 Antibody (NB500-253)(3)

Reviews for NOD2 Antibody (NB500-253) (0)

There are no reviews for NOD2 Antibody (NB500-253). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for NOD2 Antibody (NB500-253): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOD2 Antibody (NB500-253). (Showing 1 - 1 of 1 FAQs).

  1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
    • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 ( This antibody has been validated for use in WB, IHC-Paraffin and IP.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB500-253AF405
Alexa Fluor (R) 488 Labeled NB500-253AF488
Alexa Fluor (R) 647 Labeled NB500-253AF647
Alexa Fluor (R) 700 Labeled NB500-253AF700
Biotin Labeled NB500-253B
DyLight 405 Labeled NB500-253V
DyLight 488 Labeled NB500-253G
DyLight 550 Labeled NB500-253R
DyLight 650 Labeled NB500-253C
FITC Labeled NB500-253F
HRP Labeled NB500-253H

Additional NOD2 Antibody Products

Related Products by Gene

Bioinformatics Tool for NOD2 Antibody (NB500-253)

Discover related pathways, diseases and genes to NOD2 Antibody (NB500-253). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NOD2 Antibody (NB500-253)

Discover more about diseases related to NOD2 Antibody (NB500-253).

Pathways for NOD2 Antibody (NB500-253)

View related products by pathway.

PTMs for NOD2 Antibody (NB500-253)

Learn more about PTMs related to NOD2 Antibody (NB500-253).

Research Areas for NOD2 Antibody (NB500-253)

Find related products by research area.

Blogs on NOD2.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

NOD2 - inflammatory signaling and NFkB activation
Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) is an intracellular pattern recognition receptor (PRR) that plays an important role in recognizing bacterial pathogens and initiating an immune response. As a PRR, NOD2 recogniz...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NOD2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB500-253 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought