NMDAR2B [p Tyr1336] Antibody - Azide Free

Western Blot: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Analysis of Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.

Immunohistochemistry: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Staining of Mouse dentate gyrus 48 hr post TMT treatment showing NR2B when phosphorylated at Tyr1336 in green and nuclei in blue.

• Reactivity: Mu, Rt, Hu, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NMDAR2B [p Tyr1336] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336 conjugated to KLH. Accession # Q00960
• Modification: p Tyr1336
• Specificity: Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Tyr1336 . Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:400
  • Western Blot 1:1000
• Application Notes: This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~180 kDa.
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for NMDAR2B [p Tyr1336] Antibody - Azide Free

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2B subunit of the NMDARs is phosphorylated by cyclin dependent kinase-5 (cdk5) at Ser1252 in vitro and in intact cells and Ser1252 phosphorylation upregulates NMDA receptor channel activity. The phosphorylation at this site may also play a key role in ischemic cell death. (1-6)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR2B Antibody (NB300-295)(1)

Publication using NB300-295

• Berrout L, Isokawa M. Ghrelin upregulates the phosphorylation of the GluN2B subunit of the NMDA receptor by activating GHSR1a and Fyn in the rat hippocampus. Brain Res. 2017-10-06 [PMID: 28993142] (Rat)

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 2904(Human)
  • 14812(Mouse)
  • 24410(Rat)
• Uniprot:
  • Q00960(Rat)