NMDAR2B [p Tyr1472] Antibody - Azide Free

Western Blot: NMDAR2B [p Tyr1472] Antibody [NB300-182] - Rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

• Reactivity: Hu, Mu, Rt, Hu, Bv, Ca, Ch, Pm, Ze
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NMDAR2B [p Tyr1472] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 conjugated to KLH. Accession # Q00960
• Modification: p Tyr1472
• Localization: Integral membrane protein.
• Specificity: Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Tyr1472. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Canine (100%), Chicken (100%), Primate (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western
  • Western Blot 1:1000
• Application Notes: NOTE: Immunolabeling is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at ~65kDa and ~115kDa. See Simple Western Antibody Database for Simple Western validation: Tested in mouse brain lysates; separated by size; antibody dilution of 1:10.
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28273943).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for NMDAR2B [p Tyr1472] Antibody - Azide Free

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR2B Antibody (NB300-182)(2)

Publications using NB300-182

• Zhu JM, Li KX, Cao SX et al. Increased NRG1-ErbB4 signaling in human symptomatic epilepsy. Sci Rep. 2017-12-01 [PMID: 28273943] (WB, Human)
• Hallett PJ, Spoelgen R, Hyman BT et al. Dopamine D1 activation potentiates striatal NMDA receptors by tyrosine phosphorylation-dependent subunit trafficking. J Neurosci. 2006-04-26 [PMID: 16641250] (WB, Rat)
  
  Details:
  WB: primary rat embryonic neuronal cells, Fig 7D

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 24410(Rat)
• Uniprot:
  • Q00960(Rat)