NMDAR1 Antibody (2C6.2)

Product Discontinued

NMDAR1 Antibody (2C6.2) Summary

• Immunogen: Synthetic peptide (LQNQKDTVLPRRAIEREEGQLQLCSRHRES) corresponding to the C-terminus of rat NMDA receptor subunit.
• Marker: Neuronal Marker
• Specificity: NMDAR1, C-terminal, C2 cassette.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1:100-1:500
• Application Notes: Immunohistochemistry: using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy.

Reactivity Notes

Rat

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 20mM Phosphate and 0.25M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_000832.5;NM_007327.2;NM_021569.2

Alternate Names for NMDAR1 Antibody (2C6.2)

• glutamate [NMDA] receptor subunit zeta-1
• glutamate receptor, ionotropic, N-methyl D-aspartate 1
• NMDA1
• NMDAR1glutamate [NMDA] receptor subunit zeta 1
• NMD-R1
• N-methyl-D-aspartate receptor channel, subunit zeta-1
• N-methyl-D-aspartate receptor subunit NR1
• NR1

Background

N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity. A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN1
• Uniprot:
  • Q05586(Human)