NM23-H2/NME2 Antibody (7L3A2)

Western Blot: NM23-H2/NME2 Antibody (7L3A2) [NBP3-16606] - Western blot analysis of extracts of various cell lines, using NM23-H2/NME2 Rabbit mAb (NBP3-16606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Mu, Rt
• Applications: WB
• Clone: 7L3A2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NM23-H2/NME2 Antibody (7L3A2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-152 of human NM23-H2/NME2 (P22392). QHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NME2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NM23-H2/NME2 Antibody (7L3A2)

• EC 2.7.13.3
• Histidine protein kinase NDKB
• MGC2212
• NDK B
• NDKB
• NDP Kinase B2
• NDPKB
• NDPK-B
• NM23B
• NM23-H2
• NME2
• non-metastatic cells 2, protein (NM23B) expressed in
• nucleoside diphosphate kinase B
• protein (NM23) expressed in

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NME2