Recombinant Human NM23-H1 Protein

SDS-Page: Recombinant Human NM23-H1 Protein [NBP2-52250] - 15% SDS Page (3 ug)

Product Discontinued

Recombinant Human NM23-H1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-152 of Human NM23-H1
  
  

  Source: E.coli

  Amino Acid Sequence: MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE

• Details of Functionality: Specific activity is > 1,200 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NME1
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 17.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH7.5), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human NM23-H1 Protein

• EC 2.7.4.6
• GAAD
• Granzyme A-activated DNase
• Metastasis inhibition factor nm23
• NB
• NBS
• NDK A
• NDKA
• NDP kinase A
• NDPKA
• NDPK-A
• NM23A
• NM23AWD
• NM23H1
• NM23-H1
• NME1
• non-metastatic cells 1, protein (NM23A) expressed in
• nucleoside diphosphate kinase A
• Tumor metastatic process-associated protein

Background

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for NM23-H1 Recombinant Protein (NBP2-52250)(1)

Publication using NBP2-52250

• Anantha J, Goulding SR, Tuboly E Et al. NME1 Protects Against Neurotoxin-, alpha-Synuclein- and LRRK2-Induced Neurite Degeneration in Cell Models of Parkinson's Disease Molecular neurobiology 2021-10-08 [PMID: 34623600]

Bioinformatics

• Gene Symbol: NME1