NM23-H1 Antibodies
We offer NM23-H1 Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each NM23-H1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NM23-H1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the NM23-H1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NM23-H1 polyclonal antibodies and browse our NM23-H1 monoclonal antibody catalog.
Alternate Names for NM23-H1 Antibodies
anti-NM23-H1 antibody, anti-NME1 antibody, anti-EC 2.7.4.6 antibody, anti-GAAD antibody, anti-Granzyme A-activated DNase antibody, anti-Metastasis inhibition factor nm23 antibody, anti-NB antibody, anti-NBS antibody, anti-NDK A antibody, anti-NDKA antibody, anti-NDP kinase A antibody, anti-NDPK-A antibody, anti-NDPKA antibody, anti-NM23AWD antibody, anti-non-metastatic cells 1, protein (NM23A) expressed in antibody, anti-nucleoside diphosphate kinase A antibody, anti-Tumor metastatic process-associated protein antibody
20 Results for "nm23-h1" in Primary Antibodies
HAF007). For additional reference, recombinant human NM23-H1 and recombinant human NM23-H2 (10 ng/lane) were included. A specific band was detected for NM23-H1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human NM23-H1 Monoclonal Antibody (Catalog # MAB6256) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human NM23-H1 and recombinant human NM23-H2 (10 ng/lane) were included. A specific band was detected for NM23-H1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
![Western Blot: NME1-NME2 Antibody [NBP1-80992] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Western-Blot-NBP1-80992-img0011.jpg "Western Blot: NME1-NME2 Antibody [NBP1-80992] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: NME1-NME2 Antibody [NBP1-80992] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80992-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myoctes.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0014.jpg)
![Western Blot: NME1-NME2 Antibody [NBP1-80992] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Western-Blot-NBP1-80992-img0007.jpg)
![Immunohistochemistry-Paraffin: NME1-NME2 Antibody [NBP1-80992] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0009.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0012.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0013.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0014.jpg "Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunocytochemistry-Immunofluorescence-H00004830-M02-img0007.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunohistochemistry-Paraffin-H00004830-M02-img0009.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in HeLa.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0012.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in different cell lines.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0013.jpg)
![Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunoprecipitation-H00004830-M02-img0010.jpg)
![ELISA: NM23-H1 Antibody (1D7) [H00004830-M02] - Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-ELISA-H00004830-M02-img0011.jpg)
![Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Western-Blot-NBP1-47398-img0011.jpg "Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunocytochemistry-Immunofluorescence-NBP1-47398-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunohistochemistry-Paraffin-NBP1-47398-img0007.jpg)
![Flow Cytometry: NM23-H1 Antibody (4B2) [NBP1-47398] - Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Flow-Cytometry-NBP1-47398-img0009.jpg)
![Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution.<br/><br/> Lane 1: MCF7 cell lysate (20 ug/Lane)<br/> Lane 2: PC-3M cell lysate (20 ug/Lane)<br/> Lane 3: HeLa cell lysate (20 ug/Lane)<br/> Lane 4: HepG2 cell lysate (20 ug/Lane)<br/> Lane 5: A549 cell lysate (20 ug/Lane)<br/> Lane 4: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 5: C6 cell lysate (20 ug/Lane)<br/> Lane 5: Rat brain tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 17 kDa<br/> Observed band size: 17/19 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-278202415212429.jpg "Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution.<br/><br/> Lane 1: MCF7 cell lysate (20 ug/Lane)<br/> Lane 2: PC-3M cell lysate (20 ug/Lane)<br/> Lane 3: HeLa cell lysate (20 ug/Lane)<br/> Lane 4: HepG2 cell lysate (20 ug/Lane)<br/> Lane 5: A549 cell lysate (20 ug/Lane)<br/> Lane 4: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 5: C6 cell lysate (20 ug/Lane)<br/> Lane 5: Rat brain tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 17 kDa<br/> Observed band size: 17/19 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-298202414333679.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202410484818.jpg)
![Flow Cytometry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Flow cytometric analysis of MCF7 cells labeling NM23-H1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32656, 1μg/mL) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202416372150.jpg)
![Western Blot: NM23-H1 Antibody [NBP1-30884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (NBP1-30884). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Western-Blot-NBP1-30884-img0006.jpg "Western Blot: NM23-H1 Antibody [NBP1-30884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (NBP1-30884). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody [NBP1-30884] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30884-img0002.jpg)
![Immunohistochemistry: NM23-H1 Antibody [NBP1-30884] - NM23-H1 antibody [C1C3] detects NM23-H1 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. NM23-H1 antibody [C1C3] (NBP1-30884) dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-30884_rabbit-polyclonal-nm23-h1-antibody-910202420115083.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: NM23-H1 Antibody [NBP1-30884] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30884_rabbit-polyclonal-nm23-h1-antibody-1610202419414579.jpg)
![Western Blot: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution.<br/><br/> Lane 1: HEK-293 cell lysate (20 ug/Lane)<br/> Lane 2: MCF7 cell lysate (20 ug/Lane)<br/> Lane 3: Jurkat cell lysate (20 ug/Lane)<br/> Lane 4: Raji cell lysate (20 ug/Lane)<br/> Lane 5: HeLa cell lysate (20 ug/Lane)<br/> Lane 6: K-562 cell lysate (20 ug/Lane)<br/> Lane 7: A549 cell lysate (20 ug/Lane)<br/> Lane 8: A431 cell lysate (20 ug/Lane)<br/> Lane 9: HepG2 cell lysate (20 ug/Lane)<br/> Lane 10: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 11: C6 cell lysate (20 ug/Lane)<br/> Lane 12: Mouse kidney tissue lysate (40 ug/Lane)<br/> Lane 13: Mouse liver tissue lysate (40 ug/Lane)<br/> Lane 14: Rat brain tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 17 kDa<br/> Observed band size: 17/20 kDa<br/><br/> Exposure time: 1 minute 59 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-28820241423318.jpg "Western Blot: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution.<br/><br/> Lane 1: HEK-293 cell lysate (20 ug/Lane)<br/> Lane 2: MCF7 cell lysate (20 ug/Lane)<br/> Lane 3: Jurkat cell lysate (20 ug/Lane)<br/> Lane 4: Raji cell lysate (20 ug/Lane)<br/> Lane 5: HeLa cell lysate (20 ug/Lane)<br/> Lane 6: K-562 cell lysate (20 ug/Lane)<br/> Lane 7: A549 cell lysate (20 ug/Lane)<br/> Lane 8: A431 cell lysate (20 ug/Lane)<br/> Lane 9: HepG2 cell lysate (20 ug/Lane)<br/> Lane 10: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 11: C6 cell lysate (20 ug/Lane)<br/> Lane 12: Mouse kidney tissue lysate (40 ug/Lane)<br/> Lane 13: Mouse liver tissue lysate (40 ug/Lane)<br/> Lane 14: Rat brain tissue lysate (40 ug/Lane)<br/><br/> Predicted band size: 17 kDa<br/> Observed band size: 17/20 kDa<br/><br/> Exposure time: 1 minute 59 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-29820241492692.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Immunocytochemistry analysis of HeLa cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-2920241192324.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Western-Blot-NBP2-19549-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NM23-H1 Antibody [NBP2-19549] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody [NBP2-19549] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19549-img0003.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Western-Blot-NBP2-19549-img0001.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Western-Blot-NBP2-19549-img0002.jpg)
![Western Blot: NM23-H2/NME2 Antibody [NBP2-13662] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-Western-Blot-NBP2-13662-img0004.jpg "Western Blot: NM23-H2/NME2 Antibody [NBP2-13662] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Western Blot: NM23-H2/NME2 Antibody [NBP2-13662] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-Western-Blot-NBP2-13662-img0003.jpg)
![Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC3 cell lysate](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Western-Blot-NBP2-59498-img0004.jpg "Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (5F4) [NBP2-59498] - Analysis of NME1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of NME1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Immunocytochemistry-Immunofluorescence-NBP2-59498-img0002.jpg)
![Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - Lane 1: NME1, Lane 2: NME2 Lane 3: NME3 Lane 4: NME4](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Western-Blot-NBP2-59498-img0001.jpg)
![Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution.<br/><br/> Lane 1: HEK-293 cell lysate<br/> Lane 2: MCF7 cell lysate<br/> Lane 3: Jurkat cell lysate<br/> Lane 4: HeLa cell lysate<br/> Lane 5: HepG2 cell lysate<br/> Lane 6: K-562 cell lysate<br/> Lane 7: A549 cell lysate<br/> Lane 8: Raji cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 17 kDa<br/> Observed band size: 17/20 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-288202414191531.jpg "Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution.<br/><br/> Lane 1: HEK-293 cell lysate<br/> Lane 2: MCF7 cell lysate<br/> Lane 3: Jurkat cell lysate<br/> Lane 4: HeLa cell lysate<br/> Lane 5: HepG2 cell lysate<br/> Lane 6: K-562 cell lysate<br/> Lane 7: A549 cell lysate<br/> Lane 8: Raji cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 17 kDa<br/> Observed band size: 17/20 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-2920241192318.jpg)
![Western Blot: NM23-H1 Antibody [NBP3-03013] - Analysis of extracts from normal (control) and NME1 knockout (KO) 293T cells, using NM23-H1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Knockout-Validated-NBP3-03013-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NM23-H1 Antibody [NBP3-03013] - Analysis of extracts from normal (control) and NME1 knockout (KO) 293T cells, using NM23-H1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit.")
