NLE1 Antibody - BSA Free

Western Blot: NLE1 Antibody [NBP2-98646] - Anti-NLE1 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP2-98646] - Immunochemical staining of human NLE1 in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NLE1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human NLE1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLE1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NLE1 Antibody - BSA Free

• FLJ10458
• Nle
• Notchless gene homolog
• notchless homolog 1 (Drosophila)
• notchless protein homolog 1

Background

The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway.In plants, NLE1 is crucial for normal cellular growth and development. Underexpression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLE1