Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) ...read more
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for NKX6.1 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat ...read more
NKX6.1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human/Mouse NKX6.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5857) at 15 µg/mL overnight at 4 °C. ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for NKX6.1 Antibody [Unconjugated]
Homeobox protein NK-6 homolog A
NK homeo box, family 6, member A
NK homeobox (Drosophila), family 6, A
NK homeobox, family 6, A
NK6 homeobox 1
NK6 transcription factor homolog A
NK6 transcription factor related, locus 1 (Drosophila)
NK6 transcription factor related, locus 1
NKX6Ahomeobox protein Nkx-6.1
NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39‑46 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta -cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta -cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136‑173), a DNA binding homeobox (aa 236‑295) and a Glu:Asp‑rich transactivation domain (aa 305‑337). Over aa 1‑120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.