NKX3.1 Antibody

Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse NKX3.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6475) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NKX3.1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NKX3.1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse NKX3.1
  Met1-Pro124
  Accession # P97436
• Specificity: Detects mouse NKX3.1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NKX3-1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX3.1 Antibody

• BAPX2
• BAPX2homeobox protein Nkx-3.1
• Homeobox protein NK-3 homolog A
• NK homeobox (Drosophila), family 3, A
• NK3 homeobox 1
• NK3 transcription factor homolog A
• NK3 transcription factor related, locus 1 (Drosophila)
• NK3 transcription factor related, locus 1
• NKX3.1
• NKX3.1NKX3
• NKX3-1
• NKX3A
• NKX3ANK homeobox, family 3, A

Background

Nkx3.1 (Homeobox protein NK-3 homolog A) is a 38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in mouse palatine and prostatic epithelium, and appears to be regulated by both testosterone and estrogen. Nkx3.1 apparently promotes prostate gland development, and acts as a tumor-suppressor/transcriptional repressor by interacting with HDAC-1, potentially inducing IGFBP-3 secretion, and increasing p53 acetylation and half-life. Mouse Nkx3.1 is 237 amino acids (aa) in length. It contains a DNA-binding homeodomain (aa 125-184) and undergoes ubiquitination at multiple sites. Over aa 1-124, mousen Nkx3.1 shares 77% and 48% aa identity with rat and human NKX3.1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX3-1
• Uniprot:
  • P97436()