NKp46/NCR1 Antibody (29A1.4) [Unconjugated]

Mouse splenocytes were stained with (A) Rat Anti-Mouse NKp46/NCR1 Monoclonal Antibody (Catalog # MAB22252) or (B) Rat IgG2AIsotype Control Antibody (Catalog # [[catalogNumber:MAB006) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # [[catalogNumber:F0113) and anti-Mouse NK1.1 Alexa Fluor® 488-conjugated Monoclonal Antibody ([[catalogNumber:FAB76141G). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 29A1.4
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

NKp46/NCR1 Antibody (29A1.4) [Unconjugated] Summary

• Immunogen: Murine myeloma cell line NSO-derived recombinant mouse NKp46-Fc chimera
  Glu22-Asn255
  Accession # Q8C567
• Specificity: Detects mouse NKp46/NCR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp44, rhNKp46, or rhNKp80 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: NCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp46/NCR1 Antibody (29A1.4) [Unconjugated]

• CD335 antigen
• CD335
• hNKp46
• Ly94
• LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46)
• Lymphocyte antigen 94 homolog
• MAR-1
• natural cytotoxicity triggering receptor 1
• Natural killer cell p46-related protein
• NCR1
• NK cell-activating receptor
• NKp46
• NKP46FLJ99094
• NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Background

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56^dimCD16⁺ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56^brightCD16^-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
6. Arnon, T. et al. (2004) Blood 103:664.
7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
8. Mandelboim, O. et al. (2001) Nature 409:1055.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKp46/NCR1 Antibody (MAB22252)(4)

Publications using MAB22252

• Liu, J;Si, H;Huang, D;Lu, D;Zou, S;Qi, D;Pei, X;Huang, S;Li, Z; Mechanisms of Extraorbital Lacrimal Gland Aging in Mice: An Integrative Analysis of the Temporal Transcriptome Investigative ophthalmology & visual science 2023-09-01 [PMID: 37695604] (IHC, Mouse)
• Longhuan Ma, Morgan Terrell, Josephine Brown, Abigail Castellanos Garcia, Ahmed Elshikha, Laurence Morel TLR7/TLR8 activation and susceptibility genes synergize to breach gut barrier in a mouse model of lupus Frontiers in Immunology 7/6/2023 [PMID: 37483626]
• Longhuan Ma, Morgan Terrell, Josephine Brown, Abigail Castellanos Garcia, Ahmed Elshikha, Laurence Morel TLR7/TLR8 activation and susceptibility genes synergize to breach gut barrier in a mouse model of lupus Frontiers in Immunology 2023-07-06 [PMID: 37483626] (Immunohistochemistry, Mouse)
• Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection. Immunity, 2016-07-12;45(1):131-44. 2016-07-12 [PMID: 27421702] (IHC, Human, Mouse)

Bioinformatics

• Gene Symbol: NCR1
• Uniprot:
  • Q8C567()