Nitrilase 1 Antibody (OTI3A11)

Western Blot: Nitrilase 1 Antibody (3A11) [NBP2-02462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nitrilase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nitrilase 1.

Immunocytochemistry/Immunofluorescence: Nitrilase 1 Antibody (3A11) [NBP2-02462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nitrilase 1.

Immunohistochemistry-Paraffin: Nitrilase 1 Antibody (3A11) [NBP2-02462] - Staining of paraffin-embedded pancreas tissue using anti-Nitrilase 1 mouse monoclonal antibody.

Flow Cytometry: Nitrilase 1 Antibody (3A11) [NBP2-02462] - HEK293T cells transfected with either pCMV6-ENTRY Nitrilase 1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Nitrilase 1 mouse monoclonal, and then analyzed by flow cytometry.

Immunocytochemistry/Immunofluorescence: Nitrilase 1 Antibody (3A11) [NBP2-02462] - Immunofluorescent staining of HT29 cells using anti-Nitrilase 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Nitrilase 1 Antibody (3A11) [NBP2-02462] - Staining of paraffin-embedded Kidney tissue using anti-Nitrilase 1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.87 mg/ml

Nitrilase 1 Antibody (OTI3A11) Summary

• Immunogen: Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NIT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 35.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.87 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nitrilase 1 Antibody (OTI3A11)

• EC 3.5
• MGC57670
• nitrilase 1
• nitrilase homolog 1

Background

Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NIT1
• Entrez:
  • 4817(Human)